蛋白4.1R基因敲除小鼠的引种及繁殖  

Introducing and breeding of protein 4.1R gene knock out mice

作　　者：周晴晴[1] 闫红霞[1] 孙蕾[1] 刘喜龙[1] 李黎[1] 汲翔[2] 高艳锋[1] 汲振余 祁元明[1] 康巧珍[1] 

机构地区：[1]郑州大学生物工程系,郑州450001 [2]郑州大学基础医学院,郑州450001 [3]河南省医药科学研究院,郑州450052

出　　处：《郑州大学学报（医学版）》2011年第2期179-182,共4页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目30972707,30873002;河南省科技创新杰出青年基金资助项目104100510004

摘　　要：目的:引种、繁殖4.1R基因敲除小鼠,用于蛋白4.1R功能的研究。方法:按照国家进出境动物检验检疫的具体要求,对引种自美国的3对4.1R基因敲除小鼠进行隔离、检疫。引进的种鼠按照SPF动物饲养标准操作规程在IVC屏障系统中进行1雄1雌长期同居方式繁殖,C57BL/6J对照组小鼠采用同样方式饲养繁殖。定期观察记录种鼠的繁殖率、仔鼠出生率及成活率。比较基因敲除小鼠与野生型小鼠体质量变化。提取仔鼠尾组织基因组DNA,用PCR法进行基因型鉴定。结果:经隔离检疫,3对4.1R敲除小鼠符合我国SPF小鼠的微生物控制级别。23只繁殖仔鼠基因型PCR鉴定结果为基因敲除纯合子,雌鼠产仔率100%,平均胎产仔5.8只,仔鼠成活率74%。基因敲除小鼠平均体质量与野生型小鼠比较差异无统计学意义(F组间=4.035,P=0.056;F时间=543.723,P<0.001;F交互=4.358,P=0.004)。结论:在国内成功引种和繁殖C57BL/6J-4.1R-/-小鼠。Aim：To introduce and reproduce 4.1R gene knock out mouse for exploring the functions of protien 4.1R.Methods：A total of 3 pairs of 4.1R gene knock out mice introduced from the USA were isolated and inspected according to the Animal Inspection and Quarantine Law of PR China.The introduced 4.1R knock out mice were breeding in IVC equipment according to the SPF Laboratory Animal Standard Operation Procedure.A pair of one male and one female homozygote were used to reproduce 4.1R knock out mice and C57BL/6J mice were used as control.The body weights and the rate of femal reproduction,birth and survival between gene konck out and wild type mice were recorded regularly for further analysis.The genomic DNA from the murine tail tissue was subjected to PCR for genotyping.Results：The inspection and quarantine results showed that the introduced 4.1R knock out mice were qualified for the SPF laboratory mouse grade of China.PCR results showed that 23 reproduced mice were 4.1R gene knock out homozygotes.The average litter size was 5.8 and the survival rate was 74%.Though no significant difference of weight was found between wild type and 4.1R knock out mice（Fgroups=4.035,P=0.056;Ftime=543.723,P0.001;Finter=4.358,P=0.004）.Conclusion：4.1R gene knock out mice has been introduced and reproduced successfully in China.

关 键 词：基因敲除小鼠 蛋白4.1R 

分 类 号：Q95-331[生物学—动物学]