四种真菌复合多糖抗氧化与抗肿瘤作用观察  

Antioxidant and anti-tumor effects of four kinds of combined fungus polysaccharides

作　　者：朱永飞[1] 曹磊[2] 闫素清[3] 陈慧娟[1] 贾小燕[4] 

机构地区：[1]郑州大学公共卫生学院营养与食品卫生学教研室,郑州450001 [2]陕西省疾病预防控制中心,西安710054 [3]郑州大学公共卫生学院卫生化学教研室,郑州450001 [4]郑州大学公共卫生学院社会医学与卫生管理学教研室,郑州450001

出　　处：《郑州大学学报（医学版）》2011年第2期253-256,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:观察姬松茸、灰树花、云芝和香菇四种多糖复合使用的抗氧化及抗肿瘤作用。方法:Ⅰ:48只小鼠随机分为8组(n=6),分别给予高、中、低剂量的复合多糖和4种单纯多糖及超纯水,7d后检测血清总超氧化物歧化酶(T-SOD)水平及丙二醛(MDA)含量。Ⅱ:取SMMC-7721肝癌细胞,分为9组,分别给予高、中、低剂量的复合多糖,4种单纯多糖,5-Fu和培养液处理72h,采用MTT法测定肝癌细胞的生长抑制率。结果:单纯多糖和复合多糖均有抗氧化活性和抑制肿瘤细胞生长的作用,其中高、中剂量的复合多糖抗氧化和抗肿瘤活性更佳(P<0.05)。结论:四种多糖复合使用具有更好的抗氧化作用,对肿瘤细胞生长的抑制作用更强。Aim：To observe the antioxidation and anti-tumor effects of four kinds of polysaccharides,the Agaricus blazei,Grifola frondosa,Coriolus versicolor and mushrooms polysaccharides combined used.Methods：Ⅰ：A total of 48 mice were allocated into 8 groups（n=6） and treated with low,medium,and high dose combined polysaccharides,4 kinds of polysaccharides alone and ultrapure water for 7 days.The level of total superoxide dismutase（T-SOD） and the content of malondialdehyde（MDA） were determined.Ⅱ：SMMC-7721 cells were allocated into 9 groups and treated with low,medium,and high dose combined polysaccharides,4 kinds of polysaccharides alone,5-Fu,and culture solution.After 72 h culture,the inhibition rate was calculated.Results：Combined polysaccharides and polysaccharides alone all could inhibit the levels of T-SOD and MDA and the growth of SMMC-7721 cells,among which,medium and high dose combined polysaccharides had better inhibitive effects（P0.05）.Conclusion：The compound use of these four polysaccharides has better inhibition effects on tumor cell growth and antioxidative effect.

关 键 词：复合多糖 真菌 总超氧化物歧化酶 丙二醛 抗肿瘤 小鼠 SMMC-7721细胞 

分 类 号：R979.1[医药卫生—药品]