藏红花细胞悬浮培养动力学研究  

Investigation on the Kinetics of Crocus sativus L.Cell in Suspension Culture System

作　　者：陈书安[1,2] 王晓东[1] 袁晓凡[1] 赵兵[1] 王玉春[1] 

机构地区：[1]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [2]中国生物技术发展中心,北京100036

出　　处：《生物技术通报》2011年第4期102-105,共4页Biotechnology Bulletin

基　　金：中国科学院"西部行动高新技术项目"(KGCX-YW-509)

摘　　要：基于已经建立的藏红花细胞悬浮培养体系,研究了其悬浮培养动力学。结果表明,悬浮培养时细胞的生长周期约为20 d,在20 d时生物量达到最大,为12.3 g DW/L,但是藏红花素的合成周期大约为28 d,在28 d时藏红花素的含量和产量达到最大,分别为95.8 mg/g和0.92 g/L。藏红花素的积累与细胞的生长之间的关系为半生长偶联型,为其生物反应器放大培养提供了参数等理论依据。Based on the established cell suspension culture system of Crocus sativus L.,its kinetics was investigated.The highest biomass reached 12.3 g DW/L on 20th d,and the highest crocin content and production were obtained on 28th d,both of which were 95.8 mg/g and 0.92 g/L,respectively.The process of croin biosynthesis was semi-associated with the cell growth of Crocus sativus L..This research could provide references for the scale-up culture of Crocus sativus L.cell by bioreactor.

关 键 词：藏红花 悬浮 培养体系 动力学 生物反应器 

分 类 号：S567.239[农业科学—中草药栽培]