两株真养产碱杆菌部分基因的克隆与序列分析  

Cloning and Sequence Analysis of Part Gene of Two Strains of Cupriavidus metallidurans

作　　者：姚四新[1] 赵淑秋[1] 李学斌[1] 王丽荣[1] 王宪文[1] 陈仕钧[1] 张兆沛[1] 王青[1] 刘兴友[1] 刘金华[2] 

机构地区：[1]河南科技学院动物科学学院,新乡453003 [2]中国农业大学动物医学院,北京100094

出　　处：《生物技术通报》2011年第4期158-161,共4页Biotechnology Bulletin

基　　金：国家自然科学基金项目(30972202);河南科技学院院级重点项目(2010012)

摘　　要：采用随机引物PCR技术从新建细胞培养室空气中获得两段长度414 bp及450 bp的片段。通过克隆测序及序列分析,结果表明,所测序列与真养产碱杆菌主要参考菌株的同源性分别高达79%-83%及79%-84%,由其推导的氨基酸序列与真养产碱杆菌主要参考菌株的同源性高达86.4%-89.1%,由两分离菌株所获得基因片段推导的氨基酸序列之间的同源性高达98.1%,从而确定所分离两菌株为真养产碱杆菌不同亚型的菌株。The PCR products of length of 414 bp and 450 bp were derived from air of new cell culture room by using random PCR,and were cloned into pGEM-T vector for sequencing and analysis.Results showed that the identity of the nucleotide sequence of obtained genes between HN09 and HN10 strains and other Cupriavidus metallidurans reference strains were from 79%-83% and 79%-84%,respectively,and the identity of the deduced amino acid sequences of obtained genes between HN09,HN10 strains and other Cupriavidus metallidurans reference strains were from 86.4%-89.1%.The present study indicated that these two strains may belong to different subtypes of Cupriavidus metallidurans.

关 键 词：真养产碱杆菌 随机PCR 基因克隆 序列分析 

分 类 号：Q93[生物学—微生物学] Q78