不同密度红细胞的分离及其CR1受体表达与活性的研究  

Different density red blood cell separation and the study of its CR1 in the cell membrane

作　　者：周武[1] 王淑英[1] 汪德清[1] 

机构地区：[1]解放军总医院输血科,北京100853

出　　处：《中国输血杂志》2011年第3期215-218,共4页Chinese Journal of Blood Transfusion

基　　金：2010~2011年卫生行业科研专项基金(201002005)

摘　　要：目的探讨红细胞密度(天龄)与CR1受体表达及活性的关系。方法低速离心条件(3 500 g,20 min)下,采用非连续密度梯度分离方法,将每份(10份)全血标本分为6个不同密度层次(1.085、1.088、1.089 3、1.091、1.092、1.094 g/ml)的红细胞,用流式细胞仪检测各层红细胞膜CR1受体的表达,用红细胞自然免疫粘附肿瘤细胞试验检测各层红细胞膜CR1受体的活性。结果从低密度层至高密度层红细胞,其红细胞平均体积(MCV)逐渐减小[(94.9±3.8)fl^(87.2±1.5)fl],下降了8.11%,红细胞平均血红蛋白浓度(MCHC)呈递增趋势[(282.5±17.9)g/L^(316.0±23.7)g/l],增加了11.86%;红细胞膜CR1受体表达阳性率从(73.71±13.62)%减少至(49.13±21.40)%,红细胞自然免疫粘附肿瘤细胞试验阳性率从(61.0±11.7)%减至(27.0±8.2)%,各层红细胞膜CR1受体数目、CR1受体活性及密度三者之间存在一定的相关性。结论采用分离法所得的各层红细胞能反应细胞随天龄的增加而呈现的CR1受体表达和活性的变化及细胞的物理属性变化,红细胞的密度与天龄有着密切相关性。此分离方法为研究成熟红细胞天龄与功能的相关性提供了方法学基础,有助于进一步研究红细胞代谢与功能的关系。Objective To study the relations between red blood cell density（cell age）and its expression and activity of complement receptor type 1. Methods Under the low speed eentrlfugatlon condition, we used the discontinuous density gradient separation, and divided the whole blood specimen into six fractions with different density （ 1. 085,1. 088,1. 0893, 1. 091,1. 092,1. 094g/ml）. The expression of CR1 on eel] membrane in each fraction was determinated by the Flow Cytome- try,and the activity of CR1 by erythrocyte rosette test of natural immune adhering tumor cell. Results The data indicated that the mean corpuscular volume of red blood cells with different density reduced from （ 94. 9 ±3.8 ） fl to （ 87.2 ± 1.5 ） d, downed 8.11%. But mean corpuscular hemoglobin concentration increased from. （ 282. 5± 17.9 ） g/l to （ 316. 0±23.7 ） g/l, increased by 11.86%. The positive rate of Erythroeyte CR1 receptor expression reduced from（73.71± 13.62）% to（49. 13±21.40）% ,and erythrocyte rosette test from（61.0±11.7）% to （27.0±8.2）%.Among CR1 number,CR1 activeness and the cell density,they had certain relevance in each fraction. Conclusion The separation method we used could reflect the expression and activity of complement receptor type 1 changing along with the age of ceils, and the physical property changed too. The red blood ceil density correlated with cell age tightly. This separation method provided a methodological foundation of mature red blood cells about the association of function and age, and helped to further study the relationship be- tween red blood cell metabolism and function, also helped to further study the function of stored red blood ceil metabolic changes after infusion.

关 键 词：红细胞密度 梯度分离 CR1受体 表达 活性 

分 类 号：R331.14[医药卫生—人体生理学] R446.113[医药卫生—基础医学]