用激光微束穿刺法将PAP cDNA导入油菜获得抗病毒转基因植株  

Introduction of PAP cDNA into Brassica napus L. and Acquisition of Tansgenic Plants via Laser Microbeam Puncture

作　　者：张海燕[1] 党本元[1] 周奕华[1] 王兰岚[1] 刘桂珍[1] 张丽华[1] 陈正华[1] 

机构地区：[1]中国科学院遗传研究所,北京100101

出　　处：《中国激光》1999年第11期1053-1056,共4页Chinese Journal of Lasers

基　　金：国家自然科学基金

摘　　要：采用激光微束穿刺法将损伤诱导型启动子控制之下的商陆抗病毒蛋白（PAP）CDNA导入了甘蓝型油菜（BrassicanapusL．）双低品种H165，经庆大霉素筛选，获得了抗性再生植株。PCR扩增检测及Southern杂交分析表明，PAPcDNA连同损伤诱导型启动子已稳定整合至受体基因组，转化效率为1．7％。攻毒实验表明，转基因油菜植株对供试病毒芜菁花叶叶（TuMV）具有较高抵抗能力。The PAP cDNA controlled by a wound-inducible promoter was introduced into Brassica napus via laser microbeam puncture, and plantlets resistant to Gentamycin were obtained. The transgenicity of these plantlets was verified by PCR amplification and the Southern blotting analysis, which showed that PAP cDNA together with the woundinducible promoter and a terminator have been integrated into the rape genome, with the transformation frequency being 1. 7%. The test of virus challenge showed that all these transgenic rape plants were resistant to virus TuMV mechanically inoculated in higher degree.

关 键 词：油菜 抗病毒转基因 PAPcDNA 激光微束穿刺法 

分 类 号：S634.3[农业科学—蔬菜学] TN249[农业科学—园艺学]