爪蟾卵母细胞表达hERG1b钾通道的电生理学特性研究  

Electrophysiologic characteristics of hERG1b channel expressed in Xenopus oocytes

作　　者：孙洪良[1,2] 魏晓丽[1] 华南[1] 闫海涛[1] 张树卓[1] 刘晓燕[1] 郑建全[1] 

机构地区：[1]军事医学科学院毒物药物研究所,北京100850 [2]海军机关门诊部,北京100027

出　　处：《军事医学》2011年第3期184-188,共5页Military Medical Sciences

摘　　要：目的建立人类ether-a-go-go相关基因1b(human ether-a-go-go-related gene 1b,hERG1b)钾通道在爪蟾卵母细胞上的异源性表达方法和hERG1b钾通道电流记录方法,研究hERG1b钾通道的电生理学特性。方法将编码hERG1b亚基的cRNA注入爪蟾卵母细胞中表达hERG1b钾通道,使用双电极电压钳技术记录全细胞电流,分析hERG1b钾通道电流门控动力学特征和电生理特性。结果与结论利用双电极电压钳技术,记录到hERG1b钾电流,该电流具有内向整流特性,胞外钾离子浓度升高可增大hERG1b钾通道电流幅度。与hERG1a钾通道电流相比,hERG1b钾通道电流的去激活速度明显加快,表现出快速去激活的电生理特征。Objective To study the electrophysiologic characteristics of hERG1b channel currents.Methods The coding hERG1b subunit cRNA was injected into Xenopus oocytes to express hERG1b patassium ion channels.The hERG1b channel current was recorded by using two-electrode voltage clamp technique.Results and Conclusion hERG1b current was activated in response to membrane potentials of-50 mV,which displayed an inward rectifier property.hERG1b currents reached half-maximal activation at a potential of-17 mV.Compared to hERG1a channels,the deactivation kinetics of the hERG1b channel was greatly accelerated.Increase in extracellular potassium concentration could elevate the magnitude of current of hERG1b.

关 键 词：hERG1b钾通道 非洲爪蟾 电生理特性 双电极电压钳 

分 类 号：R595.3[医药卫生—内科学]