常咯啉对豚鼠和家兔单个心肌细胞钾电流的影响(英文)  

Effects of changrolin on potassium currents in guinea pig and rabbit single heart cells

作　　者：吕灵灵[1] 

机构地区：[1]厦门大学医学院生理教研室,厦门中国361005

出　　处：《中国药理学报》1999年第11期1015-1018,共4页Acta Pharmacologica Sinica

摘　　要：目的:研究常咯啉是否对心肌细胞的钾电流有影响作用。方法:采用高阻抗密封膜片箝全细胞技术,记录分离的豚鼠和家兔心肌单个细胞的钾电流。结果:在临床用量常咯啉50μmol·L^(-1)抑制家兔心房肌细胞的瞬间外向钾电流(I_(TO))17.7％±2.4％(n=8)。但并不影响电压依赖性通道。同一剂量的常咯啉对单个家兔心室肌细胞的内向整流钾电流(I_(Kl))和单个豚鼠心室肌细胞的延迟整流钾电流(I_K)并不产生任何作用。结论:提示常咯啉具有阻制(I_(TO))的作用,而对(I_K)和(I_(Kl))无任何作用。AIM: To elucidate whether or not changrolin (CRL) modifies the potassium currents (ITO, IK, and IK1) in myocardial cells. METHODS: A tight seal whole-cell patch-clamp technique was used to record ITO, IK, and IK1 in single cells isolated from guinea pig and rabbit hearts. RESULTS: At a clinically relevant concentration, CRL 50 μmol @ L-1 inhibited the transient outward current (ITO) by 17.7 % ±2.4 % (n = 8) in rabbit atrial cells. The voltage-dependence of steady-state inactivation of ITO was not affected by CRL. This concentration of CRL did not influence the time-independent inward rectifier or the delayed rectifier K+ currents (IK1 and IK, respectively) in rabbit and guinea pig ventricular cells. CONCLUSION: CRL inhibited ITO, but not IK nor IK1.

关 键 词：钾电流 常咯啉 抗心律失常药 心肌细胞 电生理学 

分 类 号：R972.2[医药卫生—药品]