耐头孢他啶阴沟肠杆菌β-内酰胺酶基因分析  

β-lactamase gene analysis of ceftazidime-resistant Enterobacter cloacae

作　　者：李巧云[1,2] 蔡挺[2] 常燕子[3] 张顺[2] 

机构地区：[1]宁波大学医学院,浙江宁波315211 [2]浙江省宁波市第二医院,浙江宁波315010 [3]浙江省宁波市医疗中心李惠利医院,浙江宁波315040

出　　处：《中国卫生检验杂志》2011年第4期896-898,904,共4页Chinese Journal of Health Laboratory Technology

基　　金：浙江省医药卫生科学研究基金项目(2009A184);宁波市农业与社发择优委托项目(2010C50038)

摘　　要：目的:分析临床分离耐头孢他啶阴沟肠杆菌的耐药性及常见β-内酰胺酶基因携带情况。方法:收集临床分离的阴沟肠杆菌,体外测试其对常用抗菌药物的敏感情况,PCR方法检测TEM等9种β-内酰胺酶基因。结果:42株耐头孢他啶阴沟肠杆菌中,产ESBL菌株有34株(80.95%),非产ESBL菌株对部分抗菌药物的耐药率高于产ESBL菌株。TEM、SHV、CTX-M、DHA、EBC、CIT、KPC耐药基因检出率分别为35.7%、50.0%、19.0%、9.5%、97.6%、28.6%、2.4%,未发现携带IMP、VIM耐药基因的菌株。结论:耐头孢他啶阴沟肠杆菌β-内酰胺酶基因检出率较高,存在多种耐药机制。Objective：To analyze the drug resistence and common β-lactamase gene distribution in clinical isolates resistant to ceftazidime-resistant Enterobacter cloacae.Methods： The antimicrobial susceptibility of clinical isolates of Enterobacter cloacae was tested in vitro;then nine β-lactamase genes（including the TEM） were amplified by PCR.Results： Of 42 ceftazidime-resistant Enterobacter cloacae,34（80.95%） were ESBL producer,and the resistance was higher in non-ESBL producing strains ESBL producing strains to some antibiotics.The drug-resistance gene detection rates of TEM,SHV,CTX-M,DHA,EBC,CIT,KPC were 35.7%,50.0%,19.0%,9.5%,97.6%,28.6%,2.4%.We didn′t find IMP and VIM resistance genes in these strains.Conclusion： There was a higher detection rate of β-lactamase gene in ceftazidime-resistant Enterobacter cloacae,and there were a variety of resistance mechanisms of Enterobacter cloacae.

关 键 词：阴沟肠杆菌 Β-内酰胺酶 耐药性 

分 类 号：R378.2[医药卫生—病原生物学]