2009年辽宁省虫媒病毒分离鉴定  

Isolation and identification of arboviruses in Liaoning province,2009

作　　者：吕志[1] 丁俊[2] 付士红[1] 高晓艳[1] 梁国栋[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所病毒性脑炎室传染病预防控制国家重点实验室,北京102206 [2]辽宁省疾病预防控制中心

出　　处：《疾病监测》2011年第3期179-181,192,共4页Disease Surveillance

基　　金："艾滋病和病毒性肝炎等重大传染病防治"国家科技重大专项(No.2008ZX10004-001);传染病重大专项(No.2008SKLID105);中美新发和再发传染病合作项目(No.U19-GH000004);中国疾病预防控制中心青年科研基金(No.2010A105)~~

摘　　要：目的调查辽宁省部分地区虫媒病毒的种类。方法 2009年8月,在辽宁省鞍山市、朝阳市、葫芦岛市和锦州北镇市采集蚊虫标本。蚊虫经分组研磨后接种C6/36和BHK-21细胞,连续3代观察细胞病变情况;对细胞病变阳性的分离物,进行抗原性检测及分子生物学鉴定。结果在辽宁省上述4个城市共采集蚊虫3600只,主要为中华按蚊(47.2%)、淡色库蚊(26.4%)、刺扰伊蚊(18.1%)及三带喙库蚊(6.9%)等。蚊虫标本分72组进行研磨,通过组织细胞培养法,共获得2株阳性分离物,经抗原性检测及核酸序列测定证实为Ⅰ型流行性乙型脑炎病毒。结论 2009年,从辽宁省鞍山地区的淡色库蚊及朝阳地区的刺扰伊蚊标本中分离到Ⅰ型流行性乙型脑炎病毒。Objective To investigate the distribution of arboviruses in part of Liaoning province.MethodsMosquitoes were collected from Chaoyang,Anshan,Jinzhou and Huludao cities in Liaoning province in 2009.The mosquitoes were divided into pools and the homogenates were inoculated onto C6/36 and BHK-21cells.The new isolates were identified by antigenic test and molecular biological methods.The sequences were analyzed by bioinformatics.Results Totally 3600 mosquitoes were collected from the 4 cities,including Anopheles sinensis（47.2%）,Culex pipiens pallens（26.4%）,Aedes vexans（18.1%） and Culex tritaeniorhynchus（6.9%）.The isolates produced CPE on BHK-21 cell line.Two isolate（LN0905 and LN0923） were identified as Japanese encephalitis virus（JEV）.Phylogenetic analysis showed that the 2 JEV stains were clustered into genotype Ⅰ.Conclusion Genotype Ⅰ JEV was isolated from the mosquitoes collected in Aanshan and Chaoyang in Liaoning province.The sequence of the new isolates had close phylogenetic relationship with JEV strains isolated in Shanghai in 2001.

关 键 词：虫媒病毒 流行性乙型脑炎病毒 序列分析 种系发生 

分 类 号：R373[医药卫生—病原生物学]