慢性铅中毒对大鼠学习记忆的影响  

IMPACT OF CHRONIC LEAD POISONING ON LEARNING AND MEMORY IN RATS

作　　者：刁波[1] 武琴[1] 朱以良[1] 张宜[1] 

机构地区：[1]广州军区武汉总医院医学实验科,武汉430070

出　　处：《现代预防医学》2011年第8期1417-1418,共2页Modern Preventive Medicine

摘　　要：[目的]研究不同浓度醋酸铅染毒对大鼠学习记忆的影响,探讨其可能作用机制。[方法]40只Wistar大鼠随机分为4组:正常组(双蒸水,A组),高铅浓度组(0.2%,B组),中铅浓度组(0.1%,C组),低铅浓度组(0.05%,D组),各组大鼠分别自由饮用不同浓度的醋酸铅水溶液2个月;分光光度法检测脑组织匀浆液中乙酰胆碱酯酶(AchE)的活性;Morris水迷宫法研究铅中毒对大鼠学习记忆的影响。[结果]与A组大鼠比较,随染铅剂量增加B、C、D组大鼠脑组织中AchE活性显著下降(P﹤0.01、P﹤0.05),水迷宫实验结果提示,B、C组大鼠在Morris水迷宫内寻找隐蔽平台所需的时间(潜伏期)比A组大鼠明显延长(P﹤0.01、P﹤0.05),D组大鼠具有延长趋势但与A组大鼠比较差异无统计学意义(P﹥0.05)。[结论]铅中毒能破坏记忆性神经递质乙酰胆碱的关键酶,损伤大鼠的学习记忆能力。[Objective]To research lead acetate with different concentration impact learning and memory in rats,and investigate its possible mechanism.[Methods]40 Wistar rats were divided into 4 groups randomly：normal group（distilled water,A group）,high concentration group（0.2%,B group）,middle concentration group（0.1%,C group）and low concentration group（0.05%,D group）.Each group drank lead acetate aqueous solution with different concentration 2 months.The vigor of AchE in brain tissue homogenate was detected by spectrophotometric method.Morris maze method was adopted to observe the impact on the learning disorder and memory disorder of lead poisoned rats.[Results]Compared with A group,the vigor of AchE in brain tissue of B,C and D groups significantly descended（P﹤0.01 and P﹤0.05,respectively）.In Morris maze experiment,there showed a significantly longer time for B and C group rats to reach the end point of the maze than those of A group（P﹤0.01 and P﹤0.05）;D group rats need more time to reach the end point of the maze either,but without no statistically significant（P﹥0.05）.[Conclusion]Lead poisoning could destroy the vigor of AchE to damage the capability of learning and memory in rats.

关 键 词：铅中毒 学习记忆 

分 类 号：R135.11[医药卫生—劳动卫生]