HPLC法测定芙蓉抗流感胶囊中槲皮素、山奈素、异鼠李素的含量  

Determination of Quercetin,Kaempferide and Isorhamnetin in Furong Anti-flu Capsules by HPLC

作　　者：邓亚利[1] 孙平川[2] 卢生杰[1] 

机构地区：[1]华南农业大学制药工程系,广州510642 [2]陕西省中医药研究院

出　　处：《中国药师》2011年第4期505-507,共3页China Pharmacist

摘　　要：目的:建立芙蓉抗流感胶囊中槲皮素、山奈素、异鼠李素含量测定方法。方法:采用Waters Xbridge C_(18)(250 mm×4.6 mm,5μm)色谱柱;以甲醇-0.4%磷酸水溶液为流动相梯度洗脱;流速为1.0 ml·min^(-1);检测波长为360 nm;柱温:25℃。结果:槲皮素、山奈素、异鼠李素的线性范围分别为63.4～634.0μg(r=0.999 9),70.8～708.0μg(r=0.999 8),45.8～458.0μg(r=0.999 8);加样回收率分别为98.45%、98.79%与99.16%。结论:该方法简单、快速、准确,可用于芙蓉抗流感胶囊的质量控制。Objective： To establish a method for determination of quercetin,kaempferide and isorhamnetin in Furong anti-flu cap- sules by HPLC. Method： HPLC method was set up using Waters Xbridge C18 column （250 mm ×4. 6 mm, 5 μm）. The mobile phase consisted of methanol and 0.4% phosphoric acid solution with gradient elution, and the flow rate was 1.0 ml. min-1 , the UV detection wavelength was 360 nm, and the column temperature was 25 ℃. Result： The linear range of quercetin, kaempferide and isorhamnetin was 63.4-634. 0μg （ r = 0. 999 9 ）, 70. 8-708.0μg （ r = 0. 999 8 ） and 45.8-458. 0μg （ r = 0. 999 8 ）, with the average recovery of 98.45% ,98.79% and 99. 16% ,respectively. Conclusion： The method is quick, simple and aeeurate. It can be used for the quality control of Furong anti-flu capsules.

关 键 词：芙蓉抗流感胶囊 高效液相色谱法 木芙蓉 

分 类 号：R927.2[医药卫生—药学]