异长春花苷内酰胺大孔树脂纯化研究  

Study on purification of strictosamide from Nauclea officinalis by macroporous resin

作　　者：孟兆青[1,2,3,4] 丁岗[1,2,3,4] 柳于介[1,2,3,4] 徐甲[1,2,3,4] 刘文君[1,2,3,4] 萧伟[1,2,3,4] 

机构地区：[1]江苏中康药物科技有限公司,江苏南京210017 [2]江苏康缘药业股份有限公司,江苏连云港222001 [3]中药制药过程新技术国家重点实验室,江苏连云港222001 [4]江苏省企业院士工作室,江苏连云港222001

出　　处：《中国中药杂志》2011年第8期1007-1010,共4页China Journal of Chinese Materia Medica

基　　金：国家科技重大专项(2009ZX09103-389);江苏省科技支撑社会发展项目(BE2009618)

摘　　要：目的:研究大孔树脂吸附分离纯化异长春花苷内酰胺的工艺,为异长春花苷内酰胺的工业化生产提供参考。方法:采用静态吸附-解吸方法,从10种大孔吸附树脂筛选出最佳树脂,另外采用单因素影响试验,对吸附量、水洗脱体积、乙醇洗脱浓度及体积进行了考察。结果:HPD400大孔树脂对异长春花苷内酰胺的吸附及解吸附效果最好,树脂的最佳吸附量为20.23 mg.g-1,水及30%乙醇洗脱体积为6 BV,70%乙醇洗脱体积为4 BV。结论:该法简单可行,分离效果好,对大生产有一定指导作用。Objective： To study on the purification of strictosamide from Nauclea officinalis by macroporous resin to provide reference for production. Method： The best macroporous resin was selected among 10 kinds of resins according to adsorption and desorption of the static adsorption experiments. The adsorption quantity, elution volume of water, concentration and elution volume of alcohol were determined according to the single factor experiment. Result： HPD400 was the best resin, and the best adsorption quantity was 20. 23 mg· g^-1, the elution volume of water and 30% alcohol was 6 BV, and the elution volume of 70% alcohol was 4 BV. Condusion： This technology is simple, feasible, and it can provide reference for the industrialized production.

关 键 词：胆木 异长春花苷内酰胺 大孔树脂 纯化 

分 类 号：R284.1[医药卫生—中药学]