留兰香油对脂多糖引起的大鼠肺气肿样改变及对MMP-9表达的影响  

Effect of spearmint oil on lipopolysaccharide induced emphysema-like changes and expression of matrix metalloproteinase-9

作　　者：刘君波[1] 王砚[1] 唐法娣[1] 余晨曦[1] 黄梦珊[1] 赵小京[1] 朱有法[1] 

机构地区：[1]浙江大学医学院呼吸药物研究实验室,浙江杭州310058

出　　处：《中国中药杂志》2011年第8期1054-1059,共6页China Journal of Chinese Materia Medica

基　　金：浙江省科技计划项目(2008c23066)

摘　　要：目的:观察留兰香油对脂多糖(LPS)所致大鼠肺气肿样改变及其对肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)和组织抑制因子-1(TIMP-1)含量及基质金属蛋白酶-9(MMP-9)表达的影响。方法:反复气道内滴入脂多糖8周建立大鼠肺气肿样改变模型,设立正常对照组、模型组、地塞米松组及留兰香油10,30,100 mg.kg-1组,各给药组灌胃治疗4周。计数大鼠支气管肺泡灌洗液(BALF)中白细胞总数及其分类;肺组织病理切片行HE,AB-PAS染色,观察肺泡结构、气道炎症及杯状细胞化生,并对病理图像进行形态学测量分析;测定肺组织TNF-α,IL-1β和TIMP-1的含量及MMP-9的表达水平。结果:留兰香油10,30,100 mg.kg-1组及地塞米松组能明显减少LPS引起的肺泡破坏;留兰香油100 mg.kg-1组及地塞米松组能明显降低BALF中白细胞总数及分类中的中性粒细胞、淋巴细胞数量并抑制杯状细胞化生;地塞米松对TNF-α,IL-1β,TIMP-1及MMP-9的表达均有抑制作用;留兰香油30,100 mg.kg-1能分别降低肺组织匀浆中的TNF-α,IL-1β含量;留兰香油10,30,100 mg.kg-1对TIMP-1的表达无影响,却能显著降低肺组织中MMP-9的表达水平。结论:留兰香油对脂多糖引起的大鼠肺气肿样改变有一定的保护作用,其机制可能是留兰香油降低了肺组织TNF-α及IL-1β的含量,抑制了MMP-9的过表达。Objective： To investigate the effect of spearmint oil on emphysema-like changes and the expression of tumor necro- sis factor-α（ TNF-α）, interleukin-1β（ IL-1β）, matrix metalloproteinase-9 （MMP-9） and tissue inhibitor of metalloproteinase-1 （TIMP- 9） in lipopolysaccharide （LPS） treated rats. Method： Emphysematous changes model was induced by intratracheal instillation of LPS once a week for up to 8 weeks in rats. Rats were divided into control, dexamethasone（0. 3 mg · kg^-1 ）, and spearmint oil（ 10,30,100 mg · kg^-1 ） groups. Each group was treated with saline, dexamethasone, and spearmint of oil respectively for 4 weeks. Then total and different white blood cell counts in bronchoalveolar lavage fluid（BALF） were carried out. The pathologic changes of lung tissue such as alveolar structure, airway inflammation, and goblet cell metaplasia were observed by HE and AB-PAS staining. Expression of TNF-α, IL-1β, TIMP-1 and MMP-9 were measured. Result： Both spearmint and dexamethasone decreased the destruction of pulmonary alveolus. The total and different white blood cell counts in BALF including neutrophile and lymphocyte of spearmint oil 100 mg · kg^-1 and dexamethasone group were significantly reduced, and the goblet cell metaplasia was also inhibited. Dexamethasone had inhibitory effect on the expression of TNF-α, IL-1β, TIMP-1 and MMP-9 . Spearmint oil 30,100 mg · kg^-1 significantly reduced TNF-α and IL-1β respectively. Spearmint oil 10,30 and 100 mg · kg^-1 had no effect on the expression of TIMP-1, but could decrease the expression of MMP-9 significantly in lung tissues. Conclusion： Spearmint oil has protective effect on rats with emphysematous changes, since it improves alveolar destruction, pulmonary inflammation, and goblet cell metaplasia. The mechanism may include reducing TNF-α, IL-1β content and inhibiting overexpression of matrix metalloproteinase-9 in lung tissues.

关 键 词：留兰香油 肺气肿样改变 脂多糖 基质金属蛋白酶-9 

分 类 号：R285.5[医药卫生—中药学]