通过酵母双杂交筛选水稻类受体激酶CR4的胞外结合蛋白  

Screening of Extracellular Binding Proteins of Rice Receptor-like Kinase CR4 by the Yeast Two-hybrid

作　　者：姚清国[1] 周二鹏[1] 王娟[1] 张文娜[1] 王景翔[1] 

机构地区：[1]石家庄学院化工学院,河北石家庄050035

出　　处：《安徽农业科学》2011年第8期4427-4430,共4页Journal of Anhui Agricultural Sciences

基　　金：2010年石家庄市科学技术研究与发展项目(10120803);2007年石家庄学院科研启动基金立项项目(2007012);2008年度石家庄学院教学改革研究项目(2008006)

摘　　要：[目的]寻找CR4的胞外结合蛋白。[方法]采用OsCR4的胞外域作为诱饵蛋白,酵母双杂交筛选14 d的小苗cDNA文库。[结果]得到了许多蛋白,包括一个过氧化物B(D26484)、甲硫氨酸硫氧还原酶(ABF96078)和一个未知功能的蛋白。[结论]为研究CR4的信号转导机制提供了理论依据。[Objective] The research aimed to find the extracellular binding proteins of CR4.[Method] The extracellular region of OsCR4 was as the bait protein,and the yeast two-hybrid was used to screen the seedling cDNA library which was cultivated 14 d.[Result] A lot of proteins which included a peroxid B（D26484）,a methionine thioredoxin reductase（ABF96078） and an unknown function protein were gained.[Conclusion]It provided the theory basis for studying the signal transduction mechanism of CR4.

关 键 词：水稻 类受体激酶 胞外结合蛋白 酵母双杂交 

分 类 号：Q291[生物学—细胞生物学]