砷钼杂多酸分光光度法测定三七中总砷含量  

Determination of As in Panax notoginseng(Burk.)F.H.Chen by Heteromolybdoarsenic Acid Spectrophotometry

作　　者：陈瑞[1] 刘卫[1,2] 黄兆龙[1,2] 易中周[1,2] 闵勇[1,2] 

机构地区：[1]红河学院理学院,云南蒙自661100 [2]云南省天然药物与化学生物学重点实验室,云南蒙自661100

出　　处：《安徽农业科学》2011年第8期4534-4535,共2页Journal of Anhui Agricultural Sciences

基　　金：国家公益性项目(200810998)

摘　　要：[目的]获得快速检测三七中总砷的方法。[方法]采用砷钼杂多酸分光光度法测定人工种植三七中的总砷含量,并优化其显色条件。[结果]最佳显色条件:显色剂用量为5.0 ml,显色温度为40℃,显色时间为30 min;AsⅢ标准溶液在浓度为0～0.04μg/ml时,与吸光度呈良好的线性关系(r=0.996 6);样品中未检测到As。[结论]该方法精密度高,稳定性好,可作为三七中砷含量的检测方法。[Objective] To obtain a rapid determination method for arsenic from Panax notoginseng（Burk.）F.H.Chen.[Method] Content of As was determined by heteromolybdoarsenic acid spectrophotometry,and the developing conditions were optimized.[Result] The optimum developing conditions were ：color developing agent amount of 5.0 ml,color developing temperature of 40 ℃ and developing duration of 30 min.When the concentration of standard AsⅢ solution ranged from 0 to 0.04 μg/ml,the linear relationship between concentration and absorbance was good（r=0.996 6）.Arsenic was not detected in sample.[Result] High in precision and good in stability,the method can be used as the determination method for As from Panax notoginseng（Burk.）F.H.Chen.

关 键 词：砷钼杂多酸分光光度法 三七 总砷 

分 类 号：S567.236[农业科学—中草药栽培]