丝胶对2型糖尿病大鼠海马HO-1表达的影响  

Effects of Sericine on HO-1 Expression in Hippocampus of Type 2 Diabetic Rats

作　　者：和亚强[1] 付文亮[1] 马洪伟[1] 宋成军[1] 陈志宏[1] 

机构地区：[1]承德医学院人体解剖学教研室,河北承德067000

出　　处：《中国医科大学学报》2011年第4期297-300,共4页Journal of China Medical University

基　　金：河北省教育厅项目(2006301);河北省科技厅项目(08276101D-19)

摘　　要：目的探讨丝胶对2型糖尿病大鼠海马血红素加氧酶1(HO-1)表达的影响。方法 30只雄性SD大鼠随机分为正常对照组、糖尿病模型组和丝胶治疗组,每组10只。糖尿病模型组和丝胶治疗组大鼠均采用链脲佐菌素连续腹腔注射建立2型糖尿病大鼠模型,以血糖≥16.7mmol/L作为成模标准。待模型成功建立后,模型组大鼠不再作任何处理,丝胶治疗组大鼠给予丝胶(2.4g·kg-·1d-1)灌胃35d。HE染色观察海马的形态变化;分别采用Western blot和RT-PCR法检测海马HO-1蛋白及mRNA的表达。结果糖尿病模型组大鼠海马CA1区神经细胞出现明显的病理变化,HO-1蛋白及mRNA的表达明显高于正常对照组大鼠(P<0.01)。丝胶治疗组大鼠海马CA1区神经细胞的病理变化明显减轻,HO-1蛋白及mRNA的表达明显低于模型组大鼠(P<0.01,P<0.05)。结论丝胶可通过下调海马HO-1的表达,减轻糖尿病时HO-1高表达对海马神经细胞的毒性损害,发挥对糖尿病神经系统损伤的保护作用。Objective To investigate the effects of sericine on heme oxygenase-1（HO-1）expression in hippocampus of type 2 diabetic rats.Methods 30 male SD rats were randomly divided into 3 groups（n =10 respectively）：normal control group,diabetes model group and sericine treatment group.The diabetes model rats were made by continuous intraperitoneal injection of streptozotocin and blood glucose≥ 16.7mmol/L was taken as the standard of model-formation.After successfully establishing diabetes rat model,rats in diabetes model group were not treated anymore;rats in sericine treatment group were lavaged with sericine（2.4 g·kg-1·d-1）for 35 days.The morphological changes of nerve cells in hippocampus were observed by HE staining.Western Blotting and RT-PCR were respectively used to detect the expression of HO-1 protein and mRNA in hippocampus.Results In diabetes model rats,the neurons in CA1 region of hippocampus showed obvious pathological changes,the expressions of HO-1 protein and mRNA were obviously higher than normal control rats（P 0.01）.Compared with diabetes model rats,the pathological changes of neurons were alleviated significantly（P 0.01）,the expressions of HO-1 protein and mRNA in CA1 region of hippocampus in sericine-treated rats decreased significantly（P 0.05）.Conclusion Sericine can alleviated the damages of neurons in hippocampus induced by high expression of HO-1,thus has protective effects on injuries of nervous system during diabetes.

关 键 词：丝胶 糖尿病 海马 血红素加氧酶1 

分 类 号：R587.1[医药卫生—内分泌]