H-和L-铁蛋白在脑膜炎中抑制由于自由铁增高造成的脑损伤  

The Inhibitory Effect of H-and L-ferritin on Increased Free Iron-induced Brain Damage in Experimental Meningitis

作　　者：任昊[1] 翟效月[1] 

机构地区：[1]中国医科大学基础医学院组织胚胎学教研室,沈阳110001

出　　处：《中国医科大学学报》2011年第4期304-306,共3页Journal of China Medical University

基　　金：高等学校博士学科点专项科研基金(200801590016)

摘　　要：目的检测铁蛋白与自由铁在脑膜炎大鼠大脑中的改变,探讨H-和L-型铁蛋白对该炎性病变脑损伤的保护作用。方法 40只新生Wistar大鼠作脑膜炎动物模型,雌雄各半,随机分组。大脑材料分别按形态学石蜡切片和定量分析的要求进行取材。利用Ferene-S方法测脑自由铁,免疫印迹测铁蛋白的表达,共聚焦显微镜观察RNA过氧化产物8-羟基鸟苷酸荧光信号。结果与对照组比较,脑自由铁在感染组中显著上升。H-和L-铁蛋白水平同时增加,且在大脑皮质与脑铁有共存关系。RNA过氧化指标8-羟基鸟苷酸无显著增加。结论大鼠脑膜炎病变过程伴随着脑自由铁的增加,但并未发生脑的过氧化损伤。H-和L-铁蛋白的表达能够结合自由铁从而保护大脑。Objective To study the changes of ferritin and free iron in brains of meningitis,and to investigate the protective effect of ferritin against inflammatory brain injuries.Methods Forty Wistar rats were used as models of meningitis,which were divided into two groups randomly with equal number of both sexes.Brain samples were extracted according to the requirements of morphological and quantitative analysis.Cerebral free iron was measured with Ferene-S method,and expression of ferritin was measured by western blot.Confocal microscopy was applied for the detection of RNA peroxidation product 8-hydroxy-guanosine（8-OHdG）.Results Compared with control group,cerebral free iron levels in the infected brains were significantly increased,as well as H-and L-ferritin levels.Ferritin expression coexisted with cerebral iron in terms of cell types.Brain RNA peroxidation indicator of 8-OhdG didn＇t increase significantly.Conclusion Pneumococcal meningitis was associated with an increase of cerebral iron level in rats,but without an increase of brain oxidation.H-and L-ferritin could combine with the free iron and thus protected the brain from oxidative damage.

关 键 词：大鼠 自由铁 铁蛋白 脑膜炎 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]