HPLC-紫外法测定人血浆中舒芬太尼浓度  

Determination of Sufentanil Concentration in Human Plasma by HPLC with UV Detection

作　　者：吴巧玲[1] 王玲玲[1] 马虹[1] 王俊科[1] 

机构地区：[1]中国医科大学附属第一医院麻醉科,沈阳110001

出　　处：《中国医科大学学报》2011年第4期307-309,共3页Journal of China Medical University

基　　金：贝朗麻醉科学研究基金(2007)

摘　　要：目的建立高效液相色谱法-紫外检测器测定人血浆中舒芬太尼浓度的方法。方法以芬太尼为内标,采用内标法进行定量,用正己烷-无水乙醇(19:1,V/V)进行液-液萃取。采用Diamonsil C18柱(4.6mm×200mm,5μm),流动相为乙腈:0.015mol/L KH2PO4缓冲液(31:69,V/V,pH=3.0),流速1.0ml/min,监测波长为200nm。结果标准曲线在2～500ng/mL范围内线性关系良好(r=0.9996),最低检测浓度为1ng/mL,方法回收率为(99.36±2.75)%,提取回收率为(98.53±2.49)%,日内变异RSD(6.75±6.18)%,日间变异RSD(6.07±5.85)%。结论本方法简便、准确、检测浓度低,能够满足血浆中低浓度舒芬太尼的测定及临床药代动力学研究的要求。Objective To determine the concentration of sufentanil in human plasma by HPLC with ultraviolet detection.Methods Fentanyl was used as internal standard.The samples were extracted by liquid-liquid extraction method with n-hexane and ethanol（19：1,V/V）.Sufentanil in human plasma was determined by HPLC with Diamonsil C18 analysis column（4.6 mm×200 mm,5 μm）.Flow rate of the mobile phase of 0.015 mol/L potassium,acetonitrile（69：31,V/V,pH=3）was 1.0 ml/min and the wavelength of detection was 200 nm.Results Calibration curve for sufentanil was linear in the range of 2 to 500 ng/mL（r = 0.999 6）.The detection limit was 1 ng/mL.The method recovery was 99.36±2.75%.The extraction recovery was 98.53±2.49%.The intraday RSD was 6.75±6.18%,the interday RSD was 5.62± 4.48%.Conclusion This method was simple and accurate for the determination of sufentanil in human plasma and useful for clinical pharmacokinetic study of sufentanil.

关 键 词：高效液相色谱法 舒芬太尼 血浆 

分 类 号：R97[医药卫生—药品]