重度子痫前期患者胎盘组织缺氧适应相关基因的表达及意义  

Expressions and Significations of Hypoxic-adjusting Related Genes in Placenta of Severe Preeclampsia

作　　者：栾南南[1] 乔宠[1] 栾奡 何政[1] 尚涛[1] 王永来[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004 [2]凤城市中医院妇产科,辽宁凤城118000

出　　处：《中国医科大学学报》2011年第4期331-333,336,共4页Journal of China Medical University

基　　金：辽宁省教育厅高校科研计划(2008791)

摘　　要：目的探讨重度子痫前期患者胎盘组织中缺氧适应相关基因HIF-1α、PHD1、PHD2和FIH-1的表达及意义。方法采用免疫组化方法检测34例重度子痫前期患者和24例正常孕妇的胎盘组织中的HIF-1α、PHD1、PHD2和FIH-1蛋白的表达,对两组的表达水平进行比较分析。结果在重度子痫前期患者和正常孕妇的胎盘组织中HIF-1α、PHD1、PHD2和FIH-1蛋白均主要表达在合体滋养细胞和细胞滋养细胞的胞浆中。重度子痫前期组HIF-1α和PHD2蛋白表达(+++)者分别为16例(16/34)和14例(14/34),显著高于对照组2例(2/24)和2例(2/24),差异均有统计学意义(P<0.01)。PHD1和FIH-1蛋白在重度子痫前期组中表达(+++)者分别是2例(2/34)和2例(2/34),明显低于对照组14例(14/24)和13例(13/24),2组比较,差异均有统计学意义(P<0.01)。结论 HIF-1α和PHD2的蛋白在重度子痫前期患者胎盘组织中表达水平明显增高;而PHD1和FIH-1的蛋白的表达水平却明显降低。PHD1、PHD2和FIH-1可能通过抑制HIF-1α的机制参与重度子痫前期的发病。Objective To investigate the expressions and significations of HIF-1α,PHD1,PHD2 and FIH-1 in the placentas of women with severe preeclampsia.Methods Placentas were collected from 34 pregnant women with severe preeclampsia as study group and from 24 normal pregnant women as control group.The expressions of HIF-1α,PHD1,PHD2 and FIH-1 proteins were semi-quantitatively analyzed with immunohistochemical assay.Results The HIF-1α,PHD1,PHD2 and FIH-1 protein were all mainly distributed in the cytoplasm of syncytiotrophoblasts and cytotrophoblasts in the placentas of patients with severe preeclampsia and normal pregnant women.The strong positive（＋＋＋）expressions of HIF-1α and PHD2 proteins were observed in 16 cases（16/34）and 14 cases（14/34）respectively in severe preeclampsia group,significantly higher than control group（2 cases,2/24 and 2 cases,2/24）.（P 0.01,P 0.01）.The strong positive（＋＋＋）expressions of PHD1 and FIH-1 proteins were observed in 2 cases and 2 cases（2/34,2/34）respectively in severe preeclampsia group,significantly lower than those in control group（14 cases,14/24 and 13 cases,13/24）（.P 0.01,P 0.01）.Conclusion Compared with the control group,expressions of HIF-1α and PHD2 are significantly higher and those of PHD1and FIH-1 are significantly lower in placentas of patients with severe preeclampsia.PHD1,PHD2 and FIH-1 might play an important role in the pathogenesis of preeclampsia through inhibition of HIF-1α by a complex mechanism.

关 键 词：重度子痫前期 胎盘 缺氧诱导因子-1Α 缺氧诱导因子脯氨酸羟化酶 因子抑制缺氧诱导因子1 

分 类 号：R714.24[医药卫生—妇产科学]