白藜三醇对兔心肌梗死后血管新生的诱导作用  

Effect of Resveratrol on Enhancing Neovascularization After Myocardial Infarction in Rabbits

作　　者：马洁[1] 周召锋[1] 徐晤[1] 张超群[1] 张卓琦[1] 王志荣[1] 

机构地区：[1]徐州医学院附属医院心内科,江苏省徐州市221006

出　　处：《中国循环杂志》2011年第2期141-144,共4页Chinese Circulation Journal

基　　金：江苏省教育厅资助项目(08KJB320015)

摘　　要：目的:探讨白藜三醇对兔心肌梗死后血管新生的诱导作用及可能机制。方法:32只新西兰白兔随机分为假手术组、手术对照组、白藜三醇组、消心痛组,每组8只。除假手术组,其余兔建立心肌梗死模型。术后予溶剂、白藜三醇或消心痛不同药物喂养。两周后,检测各组心肌梗死面积及梗死边缘区微血管密度(microvessel density,MVD)、血管内皮生长因子-A(VEGF-A)、胰岛素样生长因子-1(IGF-1)和碱性成纤维细胞生长因子(bFGF)的表达水平。结果:白藜三醇组、消心痛组较假手术组、手术对照组能明显增加梗死边缘区微血管密度及VEGF-A、IGF-1、bFGF的表达(P<0.05),白藜三醇组、消心痛组梗死面积小于手术对照组(P<0.05)。手术对照组较假手术组VEGF-A、IGF-1表达增加(P<0.05),但bFGF表达两组之间无明显变化(P>0.05);白藜三醇组IGF-1的表达水平较消心痛组明显增加(P<0.05),VEGF-A、bFGF两组之间无统计学差异(P>0.05)。结论:在缺血缺氧环境下,机体可启动心肌梗死边缘区的血管生成,但作用强度有限;白藜三醇与消心痛均可促进心肌梗死后边缘区血管生成,有利于冠状动脉侧支循环的建立,减小心肌梗死面积;白藜三醇诱导血管发生与多种血管生长因子表达增多相关,主要与介导IGF-1高表达相关。Objective ：To explore the effect of resveratrol on enhancing neovascularization after myocardial infraction in rabbits with its possible mechanism. Methods ： A total of 32 New Zealand white rabbits were divided into 4 groups, Sham operation group, n = 8 ; the myocardial infarction model was created in rest rabbits and then divided them into 3 groups with different medications as Control group, the animals received solvent, Resveratrol group and Isosorbide dinitrate group, n = 8 in each group. Two weeks after the treatment, we examined the infarcted area, micro vessel density （MVD）, the levels of vascular endothelial growth factor-A （VEGF-A） ,insulinlike growth factor-1 （IGF-1） ,and the basic fibroblast growth factor （bFGF）in different groups of all animals. Results：The MVD,VEGF-A, IGF-1, bFGF were increased in Resveratrol group and Isosorbide dinitrate group than that in Sham operation group and in Control group, P 〈 0. 05 respectively. The infracted areas in Resveratrol group and in Isosorbide dinitrate group were less than that in Control group P 〈 0. 05. The levels of VEGF-A and IGF-1 were higher in Control group than that in Sham operation group, P 〈 0. 05, while the bFGF level was similar between those two groups, P 〉 0.05. The expression of IGF-1 in Resveratrol group was significantly higher than that in Isosorbide dinitrate group, P 〈 0. 05, while there were no statistical differences of VEGF-A and bFGF expression between those two groups,P 〉 0. 05. Conclusion ： Resveratrol and isosorbide dinitrate may promote angiogenesis after myocardial infarction at the edge of infarcted area in rabbits,it is conducive to establish the coronary collateral circulation and reduce the size of infarction. The angiogenesis effect of resveratrol is related to several vascular growth factors, especially to the high expression of IGF-1.

关 键 词：白藜三醇 胰岛素样生长因子-1 血管内皮生长因子-A 碱性成纤维细胞生长因子 血管生成 

分 类 号：R541[医药卫生—心血管疾病]