结核分枝杆菌异烟肼依赖株与耐受株的耐药性调查  

Resistance of isoniazid dependent and resistant strains of Mycobacterium tuberculosis to anti-tuberculosis drugs

作　　者：杨彩娥[1] 赵利[1] 王迪[1] 匡铁吉[1] 吴纯[1] 张伟[1] 董梅[1] 

机构地区：[1]解放军309医院检验科,北京100091

出　　处：《军医进修学院学报》2011年第5期453-454,470,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金项目(30970419)~~

摘　　要：目的调查结核分枝杆菌异烟肼的依赖株和耐受株对临床常用抗结核药物的抗药性差异,探讨其原因与意义。方法采用双向匡氏琼脂培养基分离痰结核菌并作耐药性和依赖性测定。结果依赖组菌株对8种药物的耐药率均高于耐受组菌株;依赖组中耐多药(Multidrug-Resistant,MDR)株和广泛耐药(Extensively Drug-Resistant,XDR)株的比例分别为94.4%和13.9%,均高于耐受组。结论结核分枝杆菌异烟肼依赖株94.4%为MDR株,对一线和二线抗结核药物耐药率高,容易发展为XDR株,应予高度警惕和关注。Objective To investigate the resistance of isoniazid（H） dependent and resistant Mycobacterium tuberculosis（M.tuberculosis） strains to anti-tuberculosis drugs and its mechanism.Methods M.tuberculosis strains were isolated from sputum specimens and cultured with double-phase Kuang＇s agar medium.Rifampin resistant and dependent M.tuberculosis strains were detected.Results The resistance rate of M.tuberculosis strains to 8 anti-tuberculosis drugs was higher in H-dependent M.tuberculosis strains than in H-resistant M.tuberculosis strains.The ratios of multidrug-resistant（MDR） strains and extensively drug-resistant（XDR） strains were 94.4% and 13.9%,respectively,in H-dependent M.tuberculosis strains,which were higher than those in H-resistant M.tuberculosis strains.Conclusion The MDR strains account for 94.4% of all H-dependent Mycobacterium tuberculosis strains with a high resistance rate against first-and second-line anti-tuberculosis drugs and are easy to evolve into XDR strains.Thus,sharp vigilance and attention should be paid to them.

关 键 词：分枝杆菌 结核 异烟肼 物质相关性障碍 微生物敏感性试验 

分 类 号：R446.54[医药卫生—诊断学]