一种适合膜片钳试验的新生大鼠背根神经节细胞的培养方法  

A culture method of dosal root ganglion cells from new-born rats in patch clamp experiments

作　　者：马涛[1,2] 冯泽国[1] 张宏[1] 王维[2] 陶颖[3] 

机构地区：[1]解放军总医院麻醉手术中心,北京100853 [2]第二炮兵总医院麻醉科,北京100088 [3]海军总医院保健科,北京100037

出　　处：《军医进修学院学报》2011年第5期501-503,共3页Academic Journal of Pla Postgraduate Medical School

基　　金：国家自然科学基金项目(30672028)~~

摘　　要：目的建立一种适合膜片钳实验的大鼠乳鼠背根神经节细胞的培养方法。方法在显微镜及显微外科手术器械的辅助下,分离并培养新生大鼠的背根神经元(Dorsal Root Ganglion,DRG),观察培养细胞的形态,膜片钳记录细胞静息电位水平。结果 DRG神经元细胞形态结构完整,静息电位水平正常,可记录到动作电位及钠电流。结论培养的新生大鼠DRG细胞适合膜片钳试验。Objective To establish a culture method of dorsal root ganglion（DRG） cells from rats in patch clamp experiments.Methods DRG cells from rats were isolated using microsurgical instruments under microscope and cultured.Morphology of the cultured cells was observed and their resting electric potential was recorded using patch-clamps.Results The morphological structure of cultured DRG cells was intact,their resting electric potential was normal,and the Na＋ channel current could be recorded.Conclusion Cultured DRG cells from new-born rats can be used in patch-clamp experiments.

关 键 词：背根神经节 细胞培养 膜片钳 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]