HPLC法测定大鼠肝微粒体中洛伐他汀含量的方法学研究  

Methodological Study of Content Determination of Lovastatin in Rat Liver Microsomes by HPLC

作　　者：姚卓贤[1,2] 夏宗玲[2] 于锋[1] 

机构地区：[1]中国药科大学,南京市210009 [2]江苏常州市第一人民医院药剂科,常州市213001

出　　处：《中国药房》2011年第17期1562-1563,共2页China Pharmacy

摘　　要：目的:考察测定大鼠肝微粒体中洛伐他汀含量的方法学。方法:采用高效液相色谱法,内标为地西泮,色谱柱为Ulti-mateXB-C18,流动相为乙腈-水(70∶30),流速为1.0mL·min-1,检测波长为238nm,柱温为30℃,进样量为20μL。结果:大鼠肝微粒体中洛伐他汀检测浓度的线性范围为2.5～40μmol·L-1(r=0.9983),低、中、高浓度洛伐他汀溶液的日内RSD分别为5.44%、2.89%、2.83%,日间RSD分别为5.48%、1.84%、1.91%,方法回收率分别为(109.24±3.28)%、(104.82±5.92)%、(102.17±1.92)%,绝对回收率分别为(104.65±2.93)%、(113.72±6.25)%、(101.92±1.89)%。结论:方法学考察结果表明,本方法简单易行、灵敏度高,可用于肝微粒体中洛伐他汀的含量测定。OBJECTIVE： To investigate the methology of the content determination of lovastatin in rats liver microsomes. METHODS： HPLC method was adopted with diazeparn as internal standard. The determination was performed on UltimateXB-C18 column with mobile phase consisted of acetonitrile-water （70：30） at a flow rate of 1.0 mL .min^-1. The detection wavelength was set at 238 nm and column temperature was 30 ℃. The injection volume was 20 μL. RESULTS： The calibration curves of lovastatin were linear in the range of 2.5-40 μmol L^-1 （r=0.998 3）. The intra-day RSD of lovastatin at low, medium and high concentrations were 5.44%, 2.89% and 2.83%, and the inter-day RSD were 5.48%, 1.84% and 1.91%, respectively. The relative recoveries were （109.24 ± 3.28）%, （104.82 ± 5.92）%, （102.17± 1.92）%, and the absolute recoveries were （104.65 ± 2.93）%, （113.72 ± 6.25） %, （ 101.92 ± 1.89） %, respectively. CONCLUSION： Results of methological study show that the method is simple, sensitive and suitable for the content determination of lovastatin in rat liver microsomes.

关 键 词：高效液相色谱法 洛伐他汀 大鼠 肝微粒体 含量测定 

分 类 号：R972.6[医药卫生—药品] R969[医药卫生—药学]