雷米普利拉和氯沙坦单用及联用对小鼠心肌缺血-再灌注损伤的心肌保护作用比较  

Protective Effect of Ramiprilat or Losartan Alone and Their Combination against Myocardium Ischemia-reperfusion Injury in Mice

作　　者：冉玉华[1,2] 刘新友[1] 

机构地区：[1]第四军医大学附属唐都医院药剂科,西安市710033 [2]军事医学科学院毒物药物研究所心血管药理研究室,北京市100850

出　　处：《中国药房》2011年第17期1566-1568,共3页China Pharmacy

摘　　要：目的:观察血管紧张素转换酶抑制剂(ACEI)雷米普利拉与血管紧张素Ⅱ受体拮抗药(ARB)氯沙坦单用及联用对心肌缺血-再灌注损伤模型小鼠心肌的保护作用。方法:将32只C57BL/6小鼠随机均分成4组,包括对照组(生理盐水)、氯沙坦(8mg·kg-1)组、雷米普利拉(50μg·kg-1)组和氯沙坦+雷米普利拉(8mg·kg-1+50μg·kg-1)组,建立心肌缺血-再灌注损伤模型,于再灌注前5min静脉推注给药。术中监测相关指标如动脉血压、左室重量/体重(LVW/BW)、危险区域/左室重量比(AR/LV)、心率等,之后处死小鼠,对组织进行染色观察,以心肌梗死面积/危险区域(IS/AR)值作为保护心肌组织的检测指标。结果:各组小鼠动脉血压、LVW/BW、AR/LV和心率等无显著性差异;雷米普利拉和氯沙坦单用及联用组IS/AR值分别为(21.8±2.3)%、(20.8±3.2)%和(18.4±2.4)%,与对照组(39.6±2.0)%相比具有显著性差异(P<0.05),但单用组与联用组比较未见统计学差异。结论:雷米普利拉和氯沙坦均对小鼠心肌缺血-再灌注损伤具有心肌保护作用,但2药联用作用并不优于2药单用。OBJECTIVE： To observe the protective effect of ACEI ramiprilat or ARB losartan alone and their combination against myocardium ischemia-reperfusion injury in mice. METHODS： 32 C57BL/6 mice were randomly divided into four groups ineluding control group （normal saline）, losartan group （8 mg.kg^-1）, ramiprilat group （50 μg.kg^-1） and losartan＋ramiprilat （8 mg. kg^-1＋50μg.kg^-1） group. Myocardium ischemia-reperfusion injury model was established by intravenous push for 5 min before reperfusion. Physiological index were monitored during the process, such as arterial pressure, LVW/BW, AR/LV and heart rate. Mice were sacrificed and brain tissue was observed by staining using IS/AR as index. RESULTS： There were no significant differences in arterial pressure, LVW/BW, AR/LV and heart rate. IS/AR of ramiprilat or losartan alone and their combination were （21.8 ± 2.3） %, （20.8± 3.2） % and （18.4 ± 2.4） %, respectively, which is significant different from （39.6 ± 2.0） % of control group （P〈 0.05）. There was no significant difference between single therapy group and combination therapy group.CONCLUSION： Ramiprilat and losartan have protective effect against myocardium ischemia-reperfusion injury. But combination therapy is not prior to single therapy.

关 键 词：氯沙坦 雷米普利拉 心肌缺血-再灌注损伤 小鼠 心肌保护 

分 类 号：R965[医药卫生—药理学] R972.4[医药卫生—药学]