HPLC测定九里香中脱水长叶九里香内酯的含量  

Determination of Phebalosin in Murrayae Folium et Cacumen by HPLC

作　　者：姜平川 李嘉 杨海船 张赟赟 李红 黄建猷 

机构地区：[1]广西壮族自治区中医药研究院,南宁530022

出　　处：《中国现代应用药学》2011年第4期341-344,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：广西科技厅科技攻关项目(桂科攻0815005-1-22)

摘　　要：目的建立九里香药材中脱水长叶九里香内酯的含量测定方法。方法采用高效液相色谱法,使用Agilent EsclipeXDB-C18色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇-水(55∶45),流速1 mL.min-1,检测波长332 nm,柱温25℃。结果脱水长叶九里香内酯进样浓度在20.72～207.2μg.mL-1内,与峰面积呈良好的线性关系(r=0.999 8);其平均回收率为98.41%,RSD为1.65%(n=6)。结论本法简便易行,重复性好,可用于九里香药材中脱水长叶九里香内酯的含量测定。OBJECTIVE To establish a high performance liquid chromatographic method for the determination of phebalosin in Murrayae Folium et Cacumen.METHODS The analysis was carried out on an Agilent Esclipe XDB-C18 column（150 mm×4.6 mm,5 μm）,using a mixture of 55% methanol and 45% water as the mobile phase at a flow rate of 1 mL·min-1 at 25 ℃,the wavelength for measurement was 332 nm.RESULTS The calibration curve was linear in the range of 20.72·207.2 μg·mL·1（r=0.999 8）;the average recovery was 98.41% with RSD of 1.65%（n=6）.CONCLUSION The method is simple,accurate and reproducible,and it is suitable for the determination of phebalosin in Murrayae Folium et Cacumen.

关 键 词：九里香 脱水长叶九里香内酯 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R917.101[医药卫生—中医学]