中国美利奴绵羊55L9 BAC克隆基因的筛选与序列的初步分析  

Screening and sequencing of candidate genes in Chinese merino sheep 55L9 BAC clone

作　　者：董慧芹 李峰[1] 白大章[1] 杨晓亮[1] 陈芳[1] 高剑峰[1] 

机构地区：[1]石河子大学生命科学学院,新疆石河子832000

出　　处：《江苏农业学报》2011年第2期325-328,共4页Jiangsu Journal of Agricultural Sciences

基　　金：国家科技部国际合作重大项目(2006DFB33750);国家"973"子课题(2010CB30204)

摘　　要：利用绵羊55L9 BAC(细菌人造染色体)克隆制备探针,采用噬菌斑原位杂交方法,从中国美利奴细毛羊cDNA文库中筛选与免疫相关的基因,并对筛选得到的候选基因进行测序及软件分析。初步筛出12个候选阳性克隆。经过测序和比对,推测有8个独立的序列可能与绵羊的免疫及疾病相关,其中2个基因可以在已提交的BAC序列中定位。Sheep 55L9 BAC（bacterial artificial chromosomes） clones was used to prepare radioactive probes.The probes were used to screen the immune-related gene from Chinese merino cDNA library through phage in situ hybridization.The isolated candidate positive cDNA clones were sequenced and analyzed.Twelve candidate positive clones were screenedout,of which 8 separate sequences obtained by sequencing and blasting may be immune-related genes of sheep.Two genes in the 8 separate sequences were located on the 55L9 BAC.

关 键 词：相容性组织复合体(MHC) 噬菌斑原位杂交 放射性探针标记 CDNA文库 

分 类 号：S858.26[农业科学—临床兽医学]