鸡CACNA1S基因单核苷酸多态性及其与肉质性状的关联性  

Single nucleotide polymorphisms of CACNA1S gene and its association with meat quality traits of chicken

作　　者：张军[1,2] 卢立志[1] 吕佳[1,3] 王德前[1] 沈军达[1] 陶争荣[1] 田勇[1] 杨福生 黄爱霞[1] 任晋东[1] 

机构地区：[1]浙江省农业科学院畜牧兽医研究所,浙江杭州310021 [2]温州科技职业学院,浙江温州325006 [3]乐清市畜牧兽医局,浙江乐清325600 [4]杭州萧山东海养殖有限公司,浙江杭州311228

出　　处：《江苏农业学报》2011年第2期342-346,共5页Jiangsu Journal of Agricultural Sciences

基　　金：浙江省重大科技攻关项目(2005C12005-01);杭州市重点科技攻关项目(20080632B30);萧山区招投标项目(2007201);温州市科技计划项目(N20100013)

摘　　要：利用PCR-SSCP技术,对萧山鸡、仙居鸡、灵昆鸡、萧山鸡×仙居鸡杂交F1代、AA鸡的L型钙离子通道α1亚单位(CACNA1S)基因(CACNA1S)5′-调控区(5′-UTR)和外显子1(exon1)进行多态性检测,结合肉质性状做关联分析。结果表明试验群体CACNA1S基因5′-UTR和exon1存在3个单核苷酸多态性(SNPs)位点与肉质性状关联,在SNPs551位点,NN和MN基因型的肌苷酸(IMP)含量极显著(P<0.01)大于MM基因型;NN和MN基因型的肌内脂肪(IMF)含量显著(P<0.05)大于MM基因型;NN基因型的谷氨酸(Glu)含量极显著(P<0.01)大于MN、MM基因型;在SNPs716位点,KK基因型的IMP和Glu含量极显著大于(P<0.01)JJ基因型;JJ基因型的IMF含量极显著大于(P<0.01)KK基因型;在SNPs1711位点,YY基因型的IMF含量显著(P<0.05)大于XY和XX基因型;YY基因型的Glu含量显著(P<0.05)大于XY和XX基因型。初步推断CACNA1S基因可能是影响鸡肉质性状(肌内脂肪含量、肌苷酸含量、谷氨酸含量)的主效基因或与主效基因连锁,并且这些突变位点可被用作分子遗传标记选择鸡的肉质性状。Single nucleotide polymorphism（SNP） of 5′-untranslated regions（5′-UTR） and expressed region 1（exon 1） of CACNA1S（calcium channel,voltage-dependent,L type,alpha 1S subunit） gene of Xiaoshan chickens,Xianju chickens,Linkun chickens,F1 offspring of Xiaoshan and Xianju chickens,and AA chickens were analyzed by PCR-SSCP,and the association analysis with meat quality traits was conducted.It was found that three SNPs of 5′-UTR and exon 1 of CACNA1S gene were associated with meat quality traits.On the locus SNPs551,inosine-5′-monophosphate（IMP）content of genotypes NN and MN was significantly higher than that of genotype MM（P0.01）,intramuscular fat（IMF）content of genotypes NN and MN was significantly higher than that of genotypes MM（P0.05）,and glutamic acid（Glu）content of genotype NN was significantly higher than that of genotypes MN and MM（P0.01）.On the locus SNPs716,IMP content and Glu content of genotype KK were significantly higher than that of genotype JJ（P0.01）,and IMF content of genotype JJ was significantly higher than that of genotype KK（P0.01）.On the locus SNPs1711,IMF content of genotype YY was significantly higher than that of genotypes XY and XX（P0.05）,and Glu content of genotype YY was significantly higher than that of genotypes XY and XX（P0.05）.It was concluded from the results that CACNA1S gene might be the major gene affecting meat quality traits（IMF,IMP,Glu contents） of chicken or link with the major gene,and the mutation locus could be used as molecular genetic markers to select for meat quality traits of chickens.

关 键 词：鸡 CACNA1S基因 PCR-SSCP 肉质性状 关联分析 

分 类 号：S831.2[农业科学—畜牧学]