用于Hib结合疫苗生产的新型候选菌株的评价  

Evaluation on a new candidate strain for Haemophilus influenzae type b conjugate vaccine production

作　　者：王伟[1] 马雷钧[2] 王月红[3] 朱为[3] 马相虎[1] 

机构地区：[1]上海生物制品研究所生物技术制品中心,200051 [2]上海生物制品研究所国际合作部,200051 [3]上海生物制品研究所第三研究室,200051

出　　处：《国际生物制品学杂志》2011年第2期61-64,共4页International Journal of Biologicals

摘　　要：目的观察b型流感嗜血杆菌（Haemophilus influertzae typeb，Hib）760705株在连续传代过程中的稳定性。方法将Hib 760705株工作种子批菌种连续传代，对第5、第8、第10代Hib培养物进行全面检测，包括培养特性（细菌培养、卫星试验）、染色镜检、生化反应，以检测第5、第8、第10代Hib的生物学特性。同时采用血清凝集试验和聚合酶链反应英膜分型方法进行b型荚膜多糖稳定性检测。结果Hib 760705株工作种子批培养物在连续传代过程中具有典型的细菌学特性，能够稳定地产生b型荚膜多糖。结论Hib 760705株有明确的来源和背景，可以稳定传代，具备作为Hib结合疫苗生产用候选菌株的条件。Objective To observe the passage stability of Haemophilus influenzae type b （l-lib） strata 760705. Methods Hib strain 760705 was cultured for 10 passages from the working seed lot, and the subcultures of the 5th, 8th and 10th passages were detected for biological charactristics comprehensively, including cultural characteristics （ bacterial culture and satellite test）, staining and microscopic examination, and biochemical reactions. Serological agglutination test and polymerase chain reaction for capsular typing were applied to confirm the generation stability of type b capsular polysaecharide. Results The subcultures of Hib strain 760705 had typical bacteriological characteristics and capability of yielding type b capsular polysaccharide. Conclusions Hib strain 760705 has a clear origin and background and can be subcuhured stably, thus suggesting that it can be a candidate strain for Hib conjugate vaccine production.

关 键 词：嗜血菌 流感 b型 连续传代 疫苗 结合 评价研究 

分 类 号：R392[医药卫生—免疫学]