普通核桃遗传多样性的AFLP分析  

Genetic Diversity of Juglans regia L. Cultivars Revealed by AFLP Analysis

作　　者：王红霞[1] 赵书岗[2] 高仪[3] 张志华[1] 玄立春[4] 

机构地区：[1]河北农业大学山区研究所,河北保定071001 [2]河北农业大学生命科学学院,河北保定071001 [3]河北农业大学园艺学院,河北保定071001 [4]河北省迁安市林业局,河北迁安064400

出　　处：《中国农业科学》2011年第7期1434-1442,共9页Scientia Agricultura Sinica

基　　金：国家"十一五"科技支撑计划项目(2006BAD01A17);河北省科学研究与发展计划项目(04220111D)

摘　　要：【目的】从分子水平上探讨普通核桃品种的遗传多样性和亲缘关系,为更有效地保护和利用这些品种资源提供科学依据。【方法】采用AFLP-银染分子标记技术,对131份核桃品种进行遗传多样性和亲缘关系分析,应用NTSYSpc2.11a分析软件对统计结果进行聚类分析。【结果】选用20对多态性高、分辨力强的EcoRⅠ/MseⅠ引物组合分别对供试材料的基因组DNA进行扩增,共获得1 643条清晰可辨的条带,其中多态性带1 512条,平均每个引物组合可检测出82.15个多态性位点,多态性位点百分率高达92.03%。得到131份材料间的相似系数范围为0.637—0.928。当相似系数为0.76时,UPGMA分析将131份资源分成8个品种群。【结论】普通核桃种质资源具有丰富遗传多样性和复杂的遗传背景。在品种群中,通过聚类分析难以将早实核桃和晚实核桃截然分开。【Objective】The genetic diversity and phylogenetic relationship of walnut cultivars were discussed at the molecular level,thus providing a scientific basis for more effective protection and use of these resources.【Method】The genetic diversity and relationship of 131 Juglans regia L.cultivars were analyzed by AFLP（amplified fragment length polymorphism）-silver staining protocol.The specific bands were counted and analyzed by NTSYSpc2.11a software.【Result】 AFLP fingerprinting of 131 Juglans regia L.cultivars with twenty pairs of EcoRⅠ/MseⅠ primers revealed a total number of 1 643 unambiguous bands,of which 1 512 ones were polymorphic and 82.15 polymorphic bands were detected by each pair of primer on average.The polymorphism frequency was 92.03%.This result showed the abundant diversities of enzyme digestion sites among Juglans regia L.cultivars.As analyzed by NTSYSpc2.11a,the similarity coefficient of 131 Juglans regia L.cultivars ranged from 0.637 to 0.928.These Juglans regia L.cultivars were divided into eight groups by UPGMA（unweighted pair group method with arithmetic aver age） based on similarity coefficient.The genetic relationship of 131 Juglans regia L.cultivars was analyzed according to the similarity coefficient.【Conclusion】 There are rich genetic diversity and complex genetic background in walnut germplasms,but it is difficult to distinguish distinctly the precocious walnut and serotinous walnut by cluster analysis.

关 键 词：核桃 AFLP 遗传多样性 亲缘关系 

分 类 号：S664.1[农业科学—果树学]