CAPN1基因遗传变异及其对牛肉质性状的效应分析  

Genetic Variation in CAPN1 Gene and Its Association with Carcass Traits in Chinese Cattle

作　　者：武秀香[1] 施雪奎[1] 吴海涛[1] 毛永江[1] 杨章平[1] 李俊雅[2] 高会江[2] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]中国农业科学院北京畜牧兽医研究所,北京100193

出　　处：《中国农业科学》2011年第7期1466-1473,共8页Scientia Agricultura Sinica

基　　金：国家科技支撑计划(2008BADB2B03-06);国家"863"计划项目(2008AA101010)

摘　　要：【目的】探索钙蛋白酶1(CAPN1)基因在中国黄牛不同群体中的变异,以及CAPN1基因作为影响牛肉质性状候选基因的可能性,寻找与牛肉质性状相关的分子标记。【方法】采用PCR-SSCP技术对雷琼牛、云南高峰牛、BMY牛与中国西门塔尔牛共367个个体CAPN1基因Exon11-Exon16与Intron21遗传变异检测,运用SPSS17.0软件中的GLM模型分析检测到的遗传变异与中国西门塔尔牛部分肉质性状的关联性。【结果】发现了CAPN1基因DNA序列中的两个突变位点A4558G和C4684T,分别检测到AA/AG/GG和CC/CT/TT 3种基因型。相关分析表明,A4558G位点GG基因型个体剪切力和大理石花纹评分显著低于AG和AA型个体(P<0.05);C4684T位点TT基因型个体剪切力显著低于CT型个体(P<0.05)。组合基因型分析表明,GGTT组合剪切力显著低于CCAA、CTAG和CTAA(P<0.05),大理石花纹评分显著低于其它基因型组合(P<0.05),脂肪颜色评分显著高于AGTT(P<0.05),其余指标间无显著差异(P<0.05)。【结论】初步推测CAPN1基因A4558G和C4684T的位点,可以作为牛肉嫩度和大理石花纹评分的遗传标记。【Objective】To explore the distribution of Calpain 1 gene（CAPN1） in Chinese south yellow cattle populations and north beef cattle populations,and to evaluate it as a candidate molecular marker associated with beef quality.【Method】PCR-SSCP method was used to detect the polymorphism of CAPN1 in 367 heads from four cattle populations,including Leiqiong,Yunnan highpump,BMY and Chinese Simmental.The association between SNP locus and some meat straits were analyzed by general linear model（GLM） in SPSS program.【Result】 Three genotypes,AA,AG,GG and CC,CT,TT,were detected at site A4558G and C4684T,respectively,with G as an advantageous gene at site A4558G,and A as an advantageous gene at site C4684T.Genotypic variance had significant association with shearing force strait and marbling score strait.The cattle with GG in site A4558G had lower shearing force and marbling score than others（P〈0.05）,while the cattle with TT in site C4684T had lower shearing force than that of CT（P〈0.05） and individuals with TT had lower former PH after rowing of acid than that of CC（P〈0.05）.Combined-genotype analysis indicated that the cattle with GGTT had lower shearing force than that of AACC,AGCT and AACT（P〈0.05） and lower score of marbling than that of the rest（P〈0.05）,while the score of fat color of these cattle were higher than others.But there were no significant associations between two sites and other straits.【Conclusion】 Site C4684T and A4558G of CAPN1 gene could be used as genetic marks for beef tenderness and marbling score traits.

关 键 词：钙蛋白酶1基因 群体遗传分析 肉质性状 SNP 

分 类 号：S823[农业科学—畜牧学]