黄瓜绿斑驳花叶病毒西瓜、甜瓜种子的带毒率和传毒率  

The Rate of Seed Contamination and Transmission of Cucumber green mottle mosaic virus in Watermelon and Melon

作　　者：吴会杰[1] 秦碧霞[2] 陈红运[3] 彭斌[1] 蔡建和[2] 古勤生[1] 

机构地区：[1]中国农业科学院郑州果树研究所,郑州450009 [2]广西农业科学院植物保护研究所,南宁530007 [3]厦门出入境检验检疫局,福建厦门361026

出　　处：《中国农业科学》2011年第7期1527-1532,共6页Scientia Agricultura Sinica

基　　金：国家现代农业产业技术体系建设专项(nycytx-36-01-03-02);广西科学基金项目(桂科青0640024)

摘　　要：【目的】加强种子带毒检测,防止黄瓜绿斑驳花叶病毒(Cucumber green mottle mosaic virus,CGMMV)通过种子传播而导致该病害的扩散和流行。【方法】以感染CGMMV的西瓜、甜瓜病株收获的种子为材料,应用DAS-ELISA检测感染CGMMV的种子带毒率和传毒率。【结果】250粒西瓜种子全部为阳性,带毒率达100%;623株西瓜幼苗14株为阳性,传毒率为2.25%。130粒甜瓜种子122粒为阳性,带毒率达93.85%;2 050株甜瓜幼苗58株为阳性,传毒率为2.83%。灵敏度检测种子带毒量在感染种子研磨样体积与健康种子研磨样体积为1/1 000时仍检测是阳性;叶片带毒量在病叶研磨样体积与健康叶研磨样体积为1/10 000时仍检测是阳性。【结论】感染CGMMV西瓜、甜瓜种子带毒率高而传毒率相对较低,说明CGMMV主要以种子表面带毒为主。灵敏度检测表明DAS-ELISA能进行大批量的种子检测。【Objective】The objective of this study is to strengthen detection of seeds which carry virus,and to prevent the spread and prevalence of Cucumber green mottle mosaic virus（CGMMV）.【Method】 The rate of contamination and seed-to-seedling transmission of CGMMV in watermelon and melon were evaluated by DAS-ELISA.The seeds detected in the study were harvested from fruits of infected watermelon and melon plants by inoculation in insect-proof plastic house.【Result】 The results showed that the contamination rate and seed-to-seedling transmission rate of CGMMV in watermelon were 100% and 2.25%,respectively,by testing 250 seeds and 623 seedlings.The contamination rate and seed-to-seedling transmission rate of melon was 93.85% and 2.83%,respectively,by analyzing 130 melon seeds and 2 050 melon seedlings.In the mixtures of CGMMV infected/ healthy seeds or leaves,the sensitivity was successfully detected at ratios of 1/1 000 and 1/10 000,respectively.【Conclusion】The contamination rate was higher than the seed-to-seedling transmission rate,which showed that the virus distributes mainly in the seed coat.The sensitivity detection indicated that DAS-ELISA could be used to detect a large number of the seeds.

关 键 词：黄瓜绿斑驳花叶病毒 西瓜 甜瓜 种子带毒率 种子传毒率 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]