肌肽对高糖诱导的人脐静脉内皮细胞凋亡的影响  

Effects of carnosine on high glucose-induced apoptosis of human umbilical vein endothelial cells

作　　者：师岩[1,2] 房绍红[3] 周宏博[1] 

机构地区：[1]哈尔滨医科大学生物化学与分子生物学教研室,黑龙江哈尔滨150081 [2]齐齐哈尔医学院生物化学教研室,黑龙江齐齐哈尔161006 [3]教育部黑龙江省部共建心肌缺血机理与诊疗技术重点实验室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2010年第6期527-530,共4页Journal of Harbin Medical University

基　　金：黑龙江省教育厅资助项目(11541120)

摘　　要：目的探讨肌肽对高糖诱导的人脐静脉内皮细胞凋亡的影响。方法体外培养细胞,通过高糖(25mmol/L)诱导细胞凋亡模型,并给予肌肽(20 mmol/L)建立高糖加肌肽组,同时设立正常糖对照组和正常糖加肌肽组。应用电镜观察细胞超微结构;AnnexinV/PI流式细胞分析法检测细胞凋亡情况。结果电镜显示25 mmol/L葡萄糖诱导细胞呈凋亡早期形态,流式细胞仪检测高糖组细胞凋亡率显著高于正常糖组(P<0.05),而高糖加20mmol/L肌肽组与高糖组相比,具有显著抑制细胞凋亡的作用(P<0.05)。结论肌肽能够抑制高糖诱导的人脐静脉内皮细胞凋亡。Objective To explore the effects of carnosine on high glucose-induced apoptosis of human umbilical vein endothelial cells（ECV304）.Methods ECV304 cells were cultured in vitro,the cellular apoptotic model was made by the high glucose（25 mmol/L）,and the group of high glucose and carnosine was administered by the addition of high glucose（25 mmol/L） and carnosine（20 mmol/L）.In addition,the control groups were set up.Cells apoptosis were detected by electron microscopy and AnnexinV/PI flow cytometry.Results Compared with the control group,high glucose induced ECV304 apoptosis under electron microscopy and AnnexinV/PI flow cytometry（P0.05）,while 20 mmol/L carnosine inhibited the apoptosis induced by high glucose significantly（P0.05）.Conclusion Carnosine could inhibit apoptosis of human umbilical vein endothelial cells induced by high glucose.

关 键 词：肌肽 高糖 人脐静脉内皮细胞 细胞凋亡 

分 类 号：R587.1[医药卫生—内分泌] Q5[医药卫生—内科学]