类成骨细胞在流体剪切应力刺激下的生物学反应  

The biological response of osteoblast-like cells under the stimulation of fluid shear stress

作　　者：刘真[1] 王丽英[2] 杜大江[3] 

机构地区：[1]哈尔滨医科大学第二临床医学院科研实验中心,黑龙江哈尔滨150081 [2]哈尔滨医科大学第二临床医学院护理质控组,黑龙江哈尔滨150081 [3]哈尔滨医科大学第二临床医学院骨科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2010年第6期549-552,共4页Journal of Harbin Medical University

基　　金：黑龙江省教育厅海外学人科研资助项目(1155h014);黑龙江省卫生厅科研课题(2010-094)

摘　　要：目的研究流体剪切应力刺激对骨组织细胞成骨活性的生物学影响。方法利用平行板流体刺激小室系统对类成骨细胞MC 3T3-E1进行流体刺激,剪切力为4.62 dyn.s/cm2,于流体应力刺激前,30 min后,60 min后采集样本,进行RT-PCR分析。结果 RT-PCR产物电泳半定量分析显示核心结合因子(Cbfa1/Runx2)的表达较流体应力刺激前显著性增高(P<0.05),且有流体刺激时间依从性,Ⅰ型胶原表达与刺激前无明显改变(P>0.05)。结论流体剪切应力刺激可以促进类成骨细胞向成骨方向进一步分化,且与Cbfa1/Runx2的表达相关联。Objective To study the biological response of osteoblast-like cells under shear flow.Methods Shear flow stimulation of 4.62 dyn·s/cm2 were applied for 0,30,60 min on osteoblast-like cell MC 3T3-E1 by flow chamber system,and then samples were collected for RT-PCR semi-quantitive analysis.Results After stimulation,there was not obvious change of Osteoponin and Collagen Type 1 expression,but the expression of Cbfa1/Runx2 was increased significantly.Conclusion The flow shear stress could enhance further differentiation of MC 3T3-E1 in the osteogenic way,and is related with Cbfa1/Runx2 expression.

关 键 词：机械刺激 成骨分化 基因表达 

分 类 号：R318.01[医药卫生—生物医学工程]