大鼠急性胰腺炎模型的建立及不同压力高压氧治疗效果的实验研究  

Development of the acute pancreatitis model of rat and effects of hyperbaric oxygen therapy with different pressures

作　　者：周树生[1] 李矗[1] 金魁[1] 刘宝[1] 

机构地区：[1]安徽省立医院ICU,合肥230001

出　　处：《中华航海医学与高气压医学杂志》2010年第6期324-328,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：安徽省卫生厅课题基金资助项目（068028）

摘　　要：目的 探讨大鼠急性胰腺炎模型的建立及采用不同压力高压氧（HBO）治疗对胰腺炎的影响.方法 40只SD大鼠按随机数字表法分组,其中8只为假手术组,8只为非治疗对照组,24只为HBO治疗组,又按所用氧压不同分为3个亚组,每亚组为8只.采用胰管结扎的方法 制备胰腺炎模型,然后分别用不同压力的HBO治疗.在造模后的第7天处死大鼠,留取胰腺标本及血液进行分析.假手术组仪进行腹壁的切开和缝合.结果 造模后第1天胰腺淀粉酶水平假手术组为（798.5±113.5）U/L,对照组为（3156.2±639.9）U/L,假手术组与对照组之间差异有统计学意义（P＜0.05）.不同压力HBO治疗7 d之后,在各炎症细胞因子方面,各HBO治疗组与对照组的差异均有统计学意义（P＜0.05）,各HBO治疗组之间IL-2、IL-6及IL-10差异有统计学意义（P＜0.05）;细胞因子TNF-α在治疗压力0.15 MPa与0.20 MPa之间以及0.15 MPa与0.25 MPa之间差异均有统计学意义（P＜0.05）,而0.20 MPa与0.25MPa组之间差异无统计学意义（P＞0.05）.胰腺病理及电镜图片显示0.25 MPa治疗压力下改善最明显.结论 HBO治疗可以显著地减少大鼠胰腺腺泡的坏死和出血,抑制血液炎症因子的活性.Objective To establish an acute pancreatitis model of rat and investigate effects of hyperbaric oxygen （HBO） therapy with different pressures on pancreatitis. Methods Forty SD rats were randomly divided into 3 groups： the sham group （ n = 8 ）, the control group （ n = 8 ） that did not receive any treatment and the HBO therapy group （ n = 24 ）, which were further divided into 3 subgroups, each consisting of 8 rats, exposed to different pressures. The acute pancreatitis rat model was developed with pancreatic duct ligation. Then, the animals were exposed to HBO with different pressures, and were sacrificed on day 7. Blood and pancreatic tissue samples were collected for analyses. The animals in the sham group were only given celiotomy incision and suture. Pathologic changes in pancreatic tissues were observed under the optic microscope and the transmission electron microscope. Concentrations of inflammatory cytokines （IL-2, IL-6,IL-10, TNF-α） were measured with ELISA. Results Amylase level in the sham group after ligation was （798. 5 ± 113.5 ） IU/L and that in the control group was （3156.2 ± 639.9 ） IU/L. Significant statistical differences could be seen between the 2 groups. Seven days after HBO therapy with different pressures,significant differences in the concentrations of inflammatory cytokines （IL-2, IL-6, IL-10, TNF-α） for the HBO therapy groups and the control group could be noted （ P〈0.05 ）.And statistical differences in the concentrations of IL-2, IL-6, IL-10, were shown in all the HBO therapy groups（P 〈0.05）. There were statistical differences in the concentrations of TNF-α between the 0.15-0.20 MPa group and 0.15-0.25 MPa group（P 〈0.05）. And no significant differences could be seen between the 0.20 MPa and 0.25 MPa groups （P 〉 0. 05 ）. Pancreatic pathology and electron microscopy indicated that most significant improvement was noted at 0. 25 MPa therapeutic pressure. Conclusions HBO therapy with a pressure of 0. 15- 0. 20 MPa or 0. 25 MPa cou

关 键 词：大鼠 急性胰腺炎 炎症细胞因子 胰腺淀粉酶 高压氧 

分 类 号：R686[医药卫生—骨科学]