唐古特大黄多糖组分1对电离辐射损伤小鼠免疫功能的保护作用  

Purified fraction 1 of polysaccharide extracted from Rheum tanguticum prevents irradiation-induced immune damage in mice

作　　者：刘琳娜[1] 郭志伟 张琰[1] 覃华[1] 韩燕[1] 刘新友[1] 

机构地区：[1]第四军医大学唐都医院药剂科 [2]新疆布尔津县人民医院

出　　处：《中国临床药理学与治疗学》2010年第11期1201-1205,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金资助项目(81073135)

摘　　要：目的:探讨唐古特大黄多糖组分1(RTP1)对电离辐射损伤小鼠免疫功能的防护作用。方法:采用昆明种小鼠,随机分为5组:正常对照组(normal control,NC)、辐射对照组(irradiation control,IC)、RTP1低剂量组(200 mg/kg)、中剂量组(400 mg/kg)和高剂量组(800 mg/kg)。采用灌胃给药方式,连续14 d。NC组和IC组则给予等量的生理盐水。第14天除NC组外,各组小鼠均接受2.0 Gy/只60Coγ射线照射1次,照射后24 h,对小鼠的非特异性免疫功能、体液免疫功能及细胞免疫功能指标进行检测。结果:与IC组比较,RTP1组小鼠的胸腺、脾脏指数、碳廓清指数和腹腔巨噬细胞吞噬功能以及脾淋巴细胞增殖能力、血清溶血素生成值以及NK细胞活性均明显增加,与IC组比较差异有统计学意义(P<0.05或P<0.01)。结论:RTP1对辐射所致的小鼠免疫功能损伤具有一定的防护作用。AIM：To investigate the protective effect of purified fraction 1 polysaccharide extracted from Rheum tanguticum on irradiation-induced immune damage in mice. METHODS：Kunming mice were randomly divided into five groups：normal group（NC）,irradiation control group（IC）,RTP1 low dose group（200 mg/kg）,middle dose group（400 mg/kg） and high dose group（800 mg/kg）.The mice in RTP1 group were poured through gastic route by RTP1 for 14 d,the mice in NC and IC group were given by 0.9% sodium chloride solution through the same way.The mice in all groups except NC group were irradiated with 2.0 Gy 60Co γ-ray on the fourteenth day.Immune index of non-specific immune function,cellular immunity and humoral immunity were detected at the 24th hour after radiation.RESULTS：Compared with IC group,the spleen index,thymus index,the rate of carbon clearance,phagocytic function of macrophage,lymphocytes proliferation,hemolysin value of blood serum and NK activity in RTP1 groups were increased markedly（P0.05 or P0.01）.CONCLUSION：RTP1 has an obvious protective effect on damage in γ-ray radiated mice.

关 键 词：唐古特大黄多糖 免疫功能 辐射损伤 60Coγ射线 

分 类 号：R147[医药卫生—公共卫生与预防医学] R965.2