人角膜基质细胞诱导人骨髓间充质干细胞分化为角膜上皮细胞的初步研究  

Study on the transdifferentiation of human mesenchymal stem cells into corneal epithelial cells induced in vitro by co-culture with human corneal stromal fibroblasts

作　　者：蒋自培[1] 温积权[1] 徐建国[1] 孔丽萍[1] 

机构地区：[1]温州医学院附属第一医院眼科,浙江温州325000

出　　处：《中华眼视光学与视觉科学杂志》2010年第6期438-440,共3页Chinese Journal Of Optometry Ophthalmology And Visual Science

基　　金：温州市科技计划项目（Y20070140）

摘　　要：目的 探讨人骨髓间充质干细胞（MSC）体外分化为角膜上皮细胞的可行性.方法 实验研究.分别取第3代人MSC和自行培养的第3代人角膜基质细胞共同培养1周,实验组在Transwell共培养体系中培养,对照组不放置Transwell小室培养.1周后,观察实验组和对照组中人MSC光镜特征、间接免疫细胞化学染色和电镜结构,对被诱导分化的细胞进行综合鉴别.结果 第3代人MSC和人角膜基质细胞在体外培养条件下均能够较快贴壁生长.两种细胞共同培养1周后,可见部分细胞形态上呈上皮细胞特征,单克隆抗体AE1染色呈阳性,电镜下可见微绒毛、桥粒和张力丝等典型上皮细胞结构特征.结论 体外培养的人MSC在人角膜基质细胞的诱导下可能会分化为人角膜上皮样细胞.Objective To explore the feasibility of the transdifferentiation of human mesenchymal stem cell （MSC） into corneal epithelial cells in vitro. Methods Experimental study.The third passage human MSC and the third passage corneal stromal fibroblasts were co-cultured in a Transwell culture system for 1 week. The human MSC were observed and identified by light microscopy, scanning and transmission electron microscopy, and comprehensive indirect immunocytochemistry staining in the induced and control groups. Results Both the third passage human MSC and the third passage corneal stromal fibroblast cells could adhere and grow rapidly.After co-culture with corneal stromal fibroblasts for 1 week, the morphology of some induced human MSC changed into epithelial-like cells. Some induced human MSC showed positive staining for monoclonal antibody AE1, which were typical epithelium cells with a large quantity of microvilli,desmosomes and tonofilaments that were observed under electron microcopy. Conclusion The data suggests that human MSC may have the potential to transdifferentiate into human corneal epithelial-like cells in vitro.

关 键 词：骨髓间充质干细胞 人 上皮细胞 角膜 细胞分化 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]