改良胃旁路术对GK大鼠的降糖作用及其机制  

Improved gastric bypass surgery decreases blood glucose levels in Goto-Kakizaki rats

作　　者：翁山耕[1] 张斌[1] 刘赵琪[1] 石铮[1] 

机构地区：[1]福建医科大学附属第一医院肝胆胰,腔镜微创外科,福建省福州市350004

出　　处：《世界华人消化杂志》2010年第35期3778-3781,共4页World Chinese Journal of Digestology

基　　金：福建省自然基金资助项目;No.2010J01152~~

摘　　要：目的:研究改良胃旁路术(gastric bypass,GBP)对非肥胖型2型糖尿病(Goto-Kakizaki,GK)大鼠的降糖效果及其机制.方法:♂GK大鼠20只,随机分为手术组和对照组,每组10只.对手术组大鼠行GBP,对照组大鼠在十二指肠球部远端0.5cm处切断吻合.测定各组术前1wk及术后8wk体质量、空腹血糖(FPG)、餐后30min血糖、血浆胰岛素(INS)、血浆胰高血糖素样肽-1(GLP-1)水平.结果:术后8wk,手术组FPG和餐后30min血糖较术前明显降低(4.013mmol/L±0.476mmol/L vs5.050mmol/L±0.395mmol/L;12.488mmol/L±1.173mmol/L vs22.790mmol/L±3.525mmol/L,均P<0.05),血浆INS和GLP-1较术前明显升高(873.630ng/L±115.920ng/L vs674.000ng/L±224.372ng/L;8508.750ng/L±l247.013ng/L vs6915.000ng/L±1566.860ng/L,均P<0.05).结论:改良GBP能降低GK大鼠血糖,其机制可能与术后GLP-1的升高,从而导致胰岛素分泌增多有关.AIM：To investigate whether improved gastric bypass （GBP） surgery decreases blood glucose levels in Goto-Kakizaki （GK） rats and to explore possible mechanisms involved. METHODS：Twenty male GK rats were randomized into two groups：one undergoing improved GBP surgery （GBP surgery group） and the other undergoing a sham operation （sham operation group）. Body weight,fasting plasma glucose （FPG）,oral glucose tolerance （OGTT）,and plasma levels of insulin and glucagon-like peptide-1 level （GLP-1） were determined at 1 week before surgery and at 8 weeks after surgery. RESULTS：After the surgery,fasting plasma glucose level decreased from （5.050 mmol/L ±0.395 mmol/L） to （4.013 mmol/L ±0.476 mmol/L）,and OGTT from （22.790 mmol/L ±3.525 mmol/L） to （12.488 mmol/L ±1.173 mmol/L） in GK rats,while plasma insulin level increased from （674.000 ng/L ±224.372 ng/L） to （873.630 ng/L ±115.920 ng/L）,and plasma GLP-1 level from （6 915.000 ng/L ±1 566.860 ng/L） to （8 508.750 ng/L ±1 247.013 ng/L）.There were significant differences in the above parameters between the two groups of rats. CONCLUSION：Improved GBP surgery can significantly improve carbohydrate tolerance in non-obese rats with type 2 diabetes possibly by increasing plasma GLP-1 level.

关 键 词：2型糖尿病 改良胃旁路术 非肥胖型2型糖尿病大鼠 血糖 血浆胰高血糖素样肽-1 

分 类 号：R656.6[医药卫生—外科学]