镧对原代神经元存活率和细胞内活性氧含量的影响  

The effects of lanthanum on survival rate and reactive oxygen species level in primary cortex neurons in vitro

作　　者：吴洁[1,2] 刘秋芳[1] 杨敬华[1] 巫生文[1] 朱爽[1] 蔡原[1] 

机构地区：[1]中国医科大学公共卫生学院卫生毒理学教研室,辽宁沈阳110001 [2]辽宁医学院公共卫生管理学院

出　　处：《毒理学杂志》2010年第6期425-427,共3页Journal of Toxicology

基　　金：国家自然科学基金资助项目(30800925)

摘　　要：目的观察不同浓度氯化镧对大脑皮层神经元存活率和细胞内活性氧含量的影响。方法体外培养大鼠大脑皮层神经元,神经元特异性烯醇化酶(NSE)免疫细胞化学法鉴定神经元纯度,四唑蓝比色实验(MTT)检测神经元存活率,DCFH-DA荧光探针法检测细胞内活性氧含量。结果镧处理组神经元存活率降低,其中1.0 mmol/L组细胞存活率接近50%;细胞内活性氧含量升高,并呈现剂量-反应关系。结论镧可使体外培养的大鼠大脑皮层神经元存活率降低,其机制可能与细胞内活性氧水平升高有关。Objective To observe the effects of different concentrations of lanthanum chloride on survival rate and ROS levels of cerebral cortex neurons.Method Cerebral cortex neurons were cultured in vitro and applied immunocytochemistry stai-ning to identify neurons.The survival rate of neuron was mediated by MTT method.Variance of ROS was detected by specific dye DCFH-DA.Results After exposed to lanthanum,the survival rates of neurons descended and about 50% at 1.0 mmol /L dose.The intracellular ROS levels were increased in does-dependent.Conclusion Lanthanum could induce injury on cortex neuron survival,and may be related to the increase of intracellular ROS content which caused mitochondrial injury.

关 键 词：镧 皮层 神经元 活性氧 

分 类 号：R994.6[医药卫生—毒理学]