花生45S rDNA和5S rDNA的染色体定位研究  

Physical Mapping of the 45S rDNA and 5S rDNA in the Peanut (Arachis hypogaea L.)

作　　者：徐延浩[1] 李立家[2] 

机构地区：[1]长江大学农学院,长江中游湿地农业教育部工程中心,湖北荆州434025 [2]武汉大学生命科学学院,植物发育生物学教育部重点实验室,武汉430072

出　　处：《武汉植物学研究》2010年第6期649-653,共5页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金资助项目(30821064,30870261);湖北省教育厅科学技术研究项目(Q20101320)

摘　　要：对四粒红和蜀花四号花生材料进行了核型分析,四粒红为2B核型,核型公式为2n=4x=40=38m+2sm(4SAT);蜀花四号为1B核型,核型公式为2n=4x=40=40 m(2SAT)。利用双色荧光原位杂交技术,对45S rDNA和5S rDNA这两个材料有丝分裂中期染色体上的物理位置进行了定位分析。定位结果表明,四粒红有6对45S rDNA位点,位于A2L、A7S、A9L、B3L、B7S、B8L(A和B分别代表基因组A和基因组B,L和S代表长臂和短臂,数字代表染色体序号,下同);2对5S rDNA位点,位于A3S和B3S;蜀花四号有5对45S rDNA位点,位于A2L、A9L、B3L、B7S、B9L;2对5S rDNA位点,位于A3S和B3S。花生的45S rDNA位点具有可变性,5S rDNA则相对保守。Karyotype analysis of two peanut cultivars,Silihong and Shuhua No.4,was conducted.The Silihong karyotype belonged to 2B and the karyotype formula was 2n=4x=40=38m＋2sm（4SAT）.The Shuhua No.4 karyotype belonged to 1B and the karyotype formula was 2n=4x= 40=40m（2SAT）.The 45S rDNA and 5S rDNA sequences were physically mapped by double color fluorescent in situ hybridization（FISH） on the mitotic metaphase chromosomes of the two cultivars.The results showed six pairs of 45S rDNA loci on A2L,A7S,A9L,B3L,B7S,and B8L（A and B stand for genomes A and B respectively,L and S represent chromosome long and short arms,and the numbers indicate chromosomes serial number）,as well as two pairs of 5S rDNA loci on A3S and B3S in Silihong,and five pairs of 45S rDNA loci on A2L,A9L,B3L,B7S,and B9L,as well as two pairs of 5S rDNA loci on A3S and B3S in Shuhua No.4.The FISH results showed that the chromosomal locality of 5S rDNA was relatively conservative,while that of 45S rDNA was variable in the peanut.

关 键 词：荧光原位杂交 RDNA 花生 核型 

分 类 号：Q943[生物学—植物学]