甘蓝型油菜A基因组候选BAC克隆的筛选及其插入片段大小分析  

Screening and Insert Size Analysis of Candidate BAC Clones from Brassica napus A Genome

作　　者：王太霞[1] 闫晓红[2] 查敏敏[1] 王力军[2] 魏文辉[2] 

机构地区：[1]河南师范大学生命科学学院,河南新乡453007 [2]中国农业科学院油料作物研究所/农业部油料作物生物学重点开放实验室,武汉430062

出　　处：《武汉植物学研究》2010年第6期660-665,共6页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金面上项目(30671312)

摘　　要：选择甘蓝型油菜A基因组10个连锁群上特有的85个SSR分子标记,合成其引物序列,采用四维PCR法筛选甘蓝型油菜-新疆野生油菜二体异附加系BAC文库,成功筛选到甘蓝型油菜A基因组39个BAC克隆,其插入片段介于50～300 kb之间,平均为120 kb。甘蓝型油菜A基因组10个连锁群BAC克隆的获得,对后续开展甘蓝型油菜A基因组染色体识别、基因染色体定位、遗传距离与物理距离间关系分析等均具有重要价值。Thirty-nine A genome specific BAC clones were successfully screened by four-dimensional PCR,with the primer sequences of eighty-five SSR markers located in the ten linkage groups of Brassica napus A genome from the BAC library of Brassica napus-Xinjiang wild rape disomic alien addition line.The thirty-nine screened BAC clones had an average insert size of 120 kb（range 50 to 300 kb）.This set of BAC clones will be of interest to A genome chromosome identification,gene locating,and relationship analysis between physical distance and genetic distance in Brassica napus.

关 键 词：甘蓝型油菜 A基因组 SSR标记 四维PCR BAC克隆 

分 类 号：S565.4[农业科学—作物学]