RAPD双引物在构建红麻连锁图谱中的应用研究  

Applied Research on Construction of a Genetic Map of Kenaf Using Two-primer RAPD Combinations

作　　者：徐建堂[1] 陈美霞[1] 谢曾荣 祁建民[1] 方平平[1] 陶爱芬[1] 林荔辉[1] 

机构地区：[1]福建农林大学作物遗传育种与综合利用省部共建教育部重点实验室,福州350002

出　　处：《武汉植物学研究》2010年第6期666-672,共7页Journal of Wuhan Botanical Research

基　　金：国家自然科学基金资助项目(30571188);国家948项目(2006G18B);国家科技支撑项目(2006BAD06B03)

摘　　要：以阿联红麻(Alian Kenaf)与福红992(Fuhong992)杂交产生的F2代作图群体为研究材料,分别应用RAPD单引物和双引物进行多态性条带扩增,并进行扩增效果的比较研究,以期为作图群体构建红麻遗传连锁图谱奠定基础。结果表明,RAPD双引物比单引物扩增出更多的多态性条带,提高了引物的利用率和多态性条带的扩增效率。RAPD and two-primer RAPD combinations were used to screen and compare for polymorphisms between Alain and Fuhong992,providing the foundation for constructing a genetic map of kenaf.The results showed that RAPD was a potential tool for constructing a genetic linkage map of kenaf.PCR reactions with two different primers tended to produce different amplification products than the single primer,so using two different primers was a convenient and economical way of improving polymorphism.

关 键 词：RAPD双引物 红麻 遗传连锁图谱 

分 类 号：Q75[生物学—分子生物学]