红菜薹新型细胞质雄性不育系的转育研究  

Study on Transfer a New Type of Cytoplasmic Male Sterility Line in Purple-Caitai

作　　者：谭远宝[1] 何丹[1] 徐跃进[1] 万正杰[1] 

机构地区：[1]华中农业大学园艺林学学院蔬菜系国家蔬菜改良中心华中分中心园艺植物生物学教育部重点实验室,武汉430070

出　　处：《武汉植物学研究》2010年第6期756-760,共5页Journal of Wuhan Botanical Research

基　　金：湖北省科技攻关项目(20066109);948项目(2008-Z26)

摘　　要：以新型甘蓝型油菜(Brassica napus L.)细胞质雄性不育系(Eru CMS)为不育源,通过远缘杂交结合回交的方法,并运用组织培养、单株选择、异地加代种植的手段成功地将该不育基因转育到了红菜薹上。经检测所得的红菜薹(Brassica campestris L.ssp.chinensis L.var.utilis Tsen et Lee.)不育系苗期遇低温叶片不黄化,不育性稳定,蜜腺正常,不育度和不育株率均为100%。A new type of cytoplasmic male sterility line of Brassica napus（Eru CMS） was taken as a sterile source.The cytoplasmic male sterility genes were successfully transferred to Purple-Caitai by distant hybridization and backcross.These methods accelerated the transformation process,such as：tissue culture,individual selection and allopatric propagation.Results showed that the cytoplasmic male sterility line of Purple-Caitai had many advantages,including non-etiolation of leaves during low environmental temperature.In addition,terility was steady,nectary was normal,and degree of pollen steritity and rate of sterile plants were 100%.

关 键 词：红菜薹 甘蓝型油菜 细胞质雄性不育系 远缘杂交 回交 

分 类 号：S634.6[农业科学—蔬菜学] S603.2[农业科学—园艺学]