气管异位移植模型大鼠气管上皮细胞对闭塞性细支气管炎的影响  

Effects of tracheal epithelial cells on obliterative bronchiolitis in a rat tracheal heterotopic transplantation model

作　　者：张瑜[1] 刘健[1] 陈昶[2] 韩彪[1] 杨振华[1] 胡学飞[2] 

机构地区：[1]兰州大学第一附属医院胸外科,甘肃省兰州市730000 [2]同济大学附属肺科医院胸外科,上海市200433

出　　处：《中国组织工程研究与临床康复》2010年第53期9893-9896,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:目前很多实验已证明气管上皮细胞是引起这种慢性炎症的关键因素,气管上皮起到免疫应答的"靶器官"作用,可激活细胞免疫和体液免疫的应答。目的:探讨在大鼠气管异位移植中气管上皮细胞对闭塞性细支气管炎发生的影响。方法:建立大鼠去上皮组织的气管异位模型。将大鼠随机分4组:①上皮组:Wistar大鼠→SD大鼠,气管模型建立不需要消化气管上皮,直接灌入培养液。②去上皮组:Wistar大鼠→SD大鼠,没有上皮细胞的Wistar大鼠气管,将气管模型移植于SD大鼠的背部皮下。③对照组1:Wistar大鼠→Wistar大鼠,气管灌入生理盐水植入皮下。④对照组2:Wistar大鼠→Wistar大鼠,将去上皮组织的气管植于皮下。结果与结论:上皮组气管内发生了闭塞,闭塞率随着移植天数的增加而增加。去上皮组气管内没有堵塞。对照组管内没有闭塞。在大鼠气管异位移植模型中得到证实,气管上皮细胞在闭塞性细支气管炎的发生发展中起重要作用。BACKGROUND：Many studies have demonstrated that tracheal epithelial cells play a key role in chronic inflammation.The tracheal epithelium plays an immune response ＂target organ＂ effects,which can activate cellular and humoral immune response.OBJECTIVE：To study the effects of tracheal epithelial cells on obliterative bronchiolitis in rat tracheal heterotopic transplantation.METHODS：The rats were prepared for tracheal heterotopic transplantation models and randomly divided into 4 groups：epithelium group：Wistar rat tracheal model（donor） subcutaneous implanted in the back of SD rat（recipient）.In the epithelium-removed group,the procedure was performed similar to epithelium group exception of this group had no epithelial cells.Control group 1：Wistar rat tracheal heterotopic transplantation model with sodium chloride,the model subcutaneous implanted in the back of Wistar rat（recipient）.Control group 2：the same way with control group 1,however,the tracheal model had no epithelial cells.RESULTS AND CONCLUSION：The epithelium group occurred occlusion,and the occlusion rate was increased with time prolonged.There were no occlusions in the epithelium-removed group and control groups.The findings confirm that tracheal epithelial cells play an important role in the occurrence of bronchiolitis obliterans in a rat heterotopic tracheal transplantation model.

关 键 词：气管异位移植 闭塞性细支气管炎 气管上皮细胞 细胞免疫 体液免疫 

分 类 号：R617[医药卫生—外科学]