3种海洋微藻ω-3脂肪酸去饱和酶基因表达影响因素分析  

Effects on expression of Omega-3 fatty acid desaturase of 3 species of marine microalgae

作　　者：张跃群[1,2] 阎生荣[1] 吕峰[1] 金洁蓉[1] 王勇军[3] 

机构地区：[1]南通农业职业技术学院,江苏南通226007 [2]南京农业大学,南京210095 [3]南通大学,江苏南通226007

出　　处：《中国农业大学学报》2011年第2期82-88,共7页Journal of China Agricultural University

基　　金：江苏省六大人才高峰项目(06C008);国家自然科学基金资助项目(30670283)

摘　　要：研究了温度、光照和酸碱度对三角褐指藻、小球藻和球等鞭金藻-ω3脂肪酸去饱和酶基因表达的影响。实时定量PCR结果表明,10和15℃的低温处理,降低了3种微藻ω-3脂肪酸去饱和酶基因的表达丰度,提高处理温度至25和30℃（三角褐指藻除外）,则促进该基因的转录;在5 000 lx的光照强度下处理48和72 h,可以提高3种藻类的ω-3脂肪酸去饱和酶基因的表达;酸碱度对3种微藻-ω3脂肪酸去饱和酶基因的表达影响与不同的物种有关,pH 5~9的培养结果显示,酸碱度对三角褐指藻影响较大,球等鞭金藻次之,而对小球藻影响较小。In present study,the effects of temperature,light and acidity on the expression of Omega-3 fatty acid desaturase of 3 species of marine microalgae including Phaeodactylum tricornutum,Chlorella vulgaris and Isochrysis galbana were detected.The results revealed that the expression of Omega-3 fatty acid desaturase of 3 species was down-regulated following treatment with lower temperature at 10 or 15 ℃,while the transcripts of the gene were up-regulated after treatment at 25 or 30 ℃（Phaeodactylum tricornutum was excluded）.An increased expression of Omega-3 fatty acid desaturase was also found when 3 species of marine microalgae were treated with light at intensity of 5 000 lx for 48 or 72 h.The results also demonstrated that the acidity produced effects on the expression of Omega-3 fatty acid desaturase of the microalgaes in association with the species.Results of culturing in pH 5-9 showed that the acidity had a greater effects on Phaeodactylum tricornutum,whereas a moderate effects on Isochrysis galbana and a smaller effects on Chlorella vulgaris.

关 键 词：微藻 Ω-3脂肪酸 去饱和酶 基因表达 

分 类 号：Q949.2[生物学—植物学]