LTP4基因超表达载体的构建及其遗传转化  

Construction and genetic transformation of over-expression vector LTP4 gene

作　　者：徐刚标[1] 申响保[1] 鲍时来[2] 张照亮[2] 

机构地区：[1]中南林业科技大学林木遗传育种实验室,湖南长沙410004 [2]中国科学院遗传与发育生物学研究所分子发育生物学重点实验室,北京100101

出　　处：《中南林业科技大学学报》2011年第3期21-25,共5页Journal of Central South University of Forestry & Technology

基　　金：国家自然科学基金项目(31070484);湖南省教育厅项目(10C1344)

摘　　要：将脂质转移蛋白(LTP4)基因插入植物表达载体pBI121—35S,构建超表达载体pBI121—35S∶∶LTP4,通过农杆菌介导法将其转入拟南芥Arabidopsis thalianaColumbia生态型品种中,获得了卡那霉素筛选的抗性植株。PCR扩增验证及RT—PCR分析表明,该基因获得超表达。Plant over-expression vector pBI121-35S∶∶LTP4,which was constructed by inserting lipid transfer proteins（LTP4） gene into plant expression vector pBI121-35S,was transformed into Arabidopsis thaliana Columbia via Agrobacterium-mediated method.And the kanamycin-resistant plants were obtained.Results from PCR amplification and RT-PCR analysis suggested that LTP4 gene had been transformed into Arabidopsis thaliana Columbia and overexpressed.

关 键 词：脂质转移蛋白(LTP4) 超表达载体 拟南芥 遗传转化 

分 类 号：Q78[生物学—分子生物学]