游离脂肪酸对人近曲肾小管上皮细胞HK-2的影响及作用机制  

Influence and Mechanism of Free Fatty Acid on Human Renal Proximal Tubular Epithelial Cells HK-2

作　　者：韩乐[1] 刘晓玲[2] 李荣山[2] 李媛[1] 郭海莲[1] 

机构地区：[1]山西医科大学,山西太原030001 [2]山西医科大学第二临床医院,山西太原030001

出　　处：《中国现代医生》2011年第12期1-3,5,共4页China Modern Doctor

基　　金：山西省留学归国人员科研基金(200372)

摘　　要：目的探讨游离脂肪酸(Free Fatty Acids,FFAs)对人近曲肾小管上皮细胞(HK-2)的影响及作用机制。方法 用含有不同浓度(0、125、250、500μmol/L)软脂酸的DMEM-F12培养人近曲肾小管上皮细胞(HK-2)在0h、24h、48h三个时段:MTT法检测不同环境下FFAs对HK-2细胞增殖的影响,流式细胞术检测FFAs对细胞凋亡的影响,免疫细胞化学法检测处理前后HK-2细胞中诱导型一氧化氮合酶(iNOS)及凋亡相关蛋白caspase8蛋白表达的变化。结果FFAs呈剂量和时间依赖性抑制HK-2细胞的增殖(P<0.05),并可诱导细胞凋亡(P<0.05)。经FFAs作用后i,NOS及Caspase8的表达增加。结论游离脂肪酸有抑制HK-2细胞增殖,促进其凋亡的作用,FFAs可能通过激活肾小管上皮细胞内的诱导型一氧化氮合酶(iN-OS),加剧肾小管上皮细胞的凋亡,而caspase8参与了其发生和发展的过程。Objective To explore the influence and function mechanism of free fatty acid （FFAs） to proximal convoluted tubules ep- ithelial cells（HK-2）. Methods People proximal convoluted tubules epithelial ceils（HK-2）,were cultured with DMEM-F12 of different concentrations of palmitic acid （0,125,250,500 μmol/L）（using defat bovine serum albumin as vector）, normal medium as control group on the 0h.24h.48h respectively：applyied four methyl azo thiazole blue（determined by MTT） colorimetric detection different environment HK-2 cell proliferation capacity;Flow cytometric intraoperativewas used to observe apoptosis influence.Immune cell chemical method before and after treatment were detected HK-2 ceils iNOS and caspase8 protein expression changes. Results A FFAs doses and time dependence inhibited promoting HK-2 cell proliferation （P〈0.05）,its apoptosis rate rised,compared with control signiilcantly increased （P〈0.05）.When the FFAs was working, and apoptosis related proteins iNOS Caspase8 expression increased. Conclusion Free fatty acid had inhibitory HK-2 cell proliferation and promoted its apoptosis rule,FFAs may through activating renal tubular epithelial cells within the iNOS,increased renal tubular epithelial cell apoptosis,but caspase8 participated in its occurrence and development process.

关 键 词：游离脂肪酸 肾小管上皮细胞 诱导型一氧化氮合酶 CASPASE8 凋亡 增殖 

分 类 号：R692.6[医药卫生—泌尿科学]