浸棒-夹心-胶体染料免疫试验检测血吸虫病患者循环抗原方法的建立及应用  

ESTABLISHMENT AND APPLICATION OF DIPSTICK SANDWICH COLLOIDAL DYE IMMUNOASSAY FOR CIRCULATING ANTIGEN DETECTION IN SCHISTOSOMIASIS PATIENTS

作　　者：谢艳辉[1] 薛纯良[1] 娄文娴[1] 

机构地区：[1]上海第二医科大学寄生虫学教研室,上海200025

出　　处：《中国寄生虫学与寄生虫病杂志》1999年第3期129-131,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的：建立检测感染宿主血清中循环抗原的简易免疫学试验方法。方法：从国产的工业染料中首次筛选出 １ 种能用于免疫显色反应的淡紫色染料莲（ Ｈ Ｆ Ｒ Ｌ），用 Ｉｇ Ｍ 类别抗日本血吸虫组合单抗 Ｄ Ｈ Ｃ 标记染料，并探索最适标记条件。建立浸棒夹心胶体染料免疫试验（ Ｄ Ｓ Ｄ Ｉ Ａ），检测宿主血清中日本血吸虫循环抗原。结果： Ｄ Ｓ Ｄ Ｉ Ａ检测日本血吸虫 Ｓ Ｅ Ａ 的最低检出极限为 ５ ｎｇ／ｍ ｌ，分别检测急性血吸虫病患者 １４ 例及慢性血吸虫患者 １１３ 例的血清，其阳性率分别为 １００％ 和 ５２．２％ ，检测正常学生血清 １１３ 例，特异性为 ９２．９％ 。该法的敏感性及特异性与ｄｏｔ Ｅ Ｌ Ｉ Ｓ Ａ 相似，且两法联合检测可提高检出率，而特异性不变，提示两法有一定的诊断互补叠加效应。标记染料室温下保存 １ ｗ ｋ 活性不变。结论： Ｄ Ｓ Ｄ Ｉ Ａ 操作简便、试剂价廉，有广泛的应用潜力。AIM:To set up a simple immunoassay for the detection of circulating antigens in host serum. METHODS: A kind of colloidal pale purple dye (HFRL) used as a staining reagent for immunoassay was first selected from the dyes produced in China and an optimum condition for labelling the dye onto the combined IgM antibodies was explored. A dipstick sandwich colloidal dye immunoassay (DS DIA) for the detection of circulating antigens in host sera was established. RESULTS:The minimal concentration of SEA detected was 5 ng/ml by DS DIA. Serum circulating antigen detection in 14 cases of acute schistosomiasis japonica ,113 chronic cases and in 113 healthy controls revealed that the sensitivity was 100% in acute cases, 52.2% in chronic cases and the specificity was 92.9% for uninfected students.The sensitivity and specificity of DIA were similar to those of dot ELISA . A certain degree of diagnostic complementarity up to 76.1% in sera from these patients was seen when DS DIA and dot ELISA were used in parallel. CONCLUSION:DS DIA is a simple, economical and reliable method for detecting circulating antigens of Schistosoma japonicum ,having a wide potential value especially for field use.

关 键 词：血吸虫病 循环抗原检测 免疫诊断 DIA 

分 类 号：R532.21[医药卫生—内科学]