杀菌/通透性增加蛋白对内毒素休克大鼠微循环灌注的保护作用  

The protective effects of recombinant amino terminal fragment of bactericidal/permeability increasing protein on systemic hemodynamics and microcirculation in a rat model of endotoxic shock

作　　者：姚咏明 彭志齐[2] 常国友 于燕 盛志勇 

机构地区：[1]解放军第三零四医院创伤外科中心,北京100037 [2]解放军工程兵工程学院医院

出　　处：《中华麻醉学杂志》1999年第7期410-413,共4页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金!No.3 9870 2 86 ;军队杰出中青年人才专项基金!No.98J0 13

摘　　要：目的　探讨杀菌 /通透性增加蛋白 ( BPI)对内毒素休克时血液动力学和内脏微循环灌注的保护作用及其机制。方法　采用大鼠内毒素休克模型 ,动态观察血液动力学、内脏微循环灌注量和血浆生物喋呤、一氧化氮 ( NO)水平的变化。结果　休克早期给予重组 BPI,可显著提高平均动脉压、心脏指数及每搏输出量 ,明显改善肝、肾、小肠微循环灌注量及动物预后 ( P<0 .0 5～ 0 .0 1) ;同时 ,治疗组血浆生物喋呤水平显著降低 ( P<0 .0 5～ 0 .0 1) ,循环 NO产生亦不同程度受到抑制。结论　早期应用 BPI能有效减轻内毒素休克时全身血液动力学紊乱及组织微循环障碍 ,该保护效应与 BPI抑制机体生物喋呤、NO的诱生相关。Objective To evaluate the potential effects of recombinant amino terminal fragment of bactericidal/permeability increasing protein(rBPI 21 )on systemic hemodynamics and internal organ microcirculation following septic shock.Methods Adult male rats were subjected to endotoxic shock induced by a bolus intravenous injection of endotoxin(10.0 mg/kg),and hemodynamic parameters,internal organ microvascular blood flow,plasma biopterin,as well as nitric oxide levels were determined in rats at various intervals.Results Early treatment with rBPI 21 significantly elevated arterial blood pressure,cardiac index,and stroke volume compared with non treated group(P<0 05 0 01) Meanwhile,microvascular blood flow in liver,kidney,as well as small intestine,and survival rate were markedly improved in animals received rBPI 21 treatment.In addition,plasma biopterin and nitric oxide levels were much lower in the treatment group than in the non treatment group (P<0 05 0 01).Conclusion During early stage after septic shock,administration of rBPI 21 can potently attenuate cardiovascular and microcirculatory dysfunction,which may be associated with its inhibitory effects on biopterin and nitric oxide synthesis evoked by endotoxin challenge.

关 键 词：脓毒性休克 血液动力学 微循环 BPI 

分 类 号：R631.4[医药卫生—外科学]