活化淋巴细胞及其染色质诱导IgG1亚类抗双链DNA抗体生成  

Induction of antidsDNA IgG1 antibody by immunization with activated lymphocytes and their active chromatin

作　　者：吴厚生[1] 奚华国[1] 卢琳[1] 王美英[1] 

机构地区：[1]上海医科大学免疫学教研室

出　　处：《中国免疫学杂志》1999年第7期291-293,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金

摘　　要：目的：抗双链ＤＮＡ（ｄｓＤＮＡ）抗体的诱生机理不明，试图研究诱导该自身抗体产生的相应自身抗原。方法：取体外活化的Ｂａｌｂ／ｃ小鼠脾细胞及其染色质免疫同系小鼠，用ＥＬＩＳＡ方法检测ＩｇＧ类抗双链ＤＮＡ抗体及其ＩｇＧ亚类。结果：不同原因活化的脾细胞及其染色质均可诱导ＩｇＧ类抗双链ＤＮＡ抗体产生，且抗体均为ＩｇＧ１亚类。结论：活化淋巴细胞及其活性染色质是诱导双链ＤＮＡ抗体生成的自身免疫原。Objective: The relevant primary autoantigen which induced antidsDNA antibodies was still enigmatic.Attempted to investigate the primary autoantigens driving the productiorn of these autoantibodies. Methods: Immunized Balb/c mice with activated syngenic spleen cells primed by ConA or LPS or rIL2 in vitro and the chromatin isolated from activated spleen cells.AntidsDNA IgG antibodies and its subclass were determined by ELISA. Results: The activated lymphocytes and its chromatin all induced antidsDNA antibodies and these autoantibodies belong to IgG1 subclass. Conclusion: The results indicated that activated lymphocytes and their active chromatin can be the autoimmunogenes driving the production of antidsDNA antibodies.

关 键 词：活化淋巴细胞 染色质 双链DNA抗体 IgG1亚类 

分 类 号：R392.3[医药卫生—免疫学]